原料乳中高产蛋白酶嗜冷菌的分离及产酶培养基的研究

张阳旸; 刘景; 陈万义; 王渊龙; 任婧

doi:10.13386/j.issn1002-0306.2014.20.036

原料乳中高产蛋白酶嗜冷菌的分离及产酶培养基的研究

1. 乳业生物技术国家重点实验室,光明乳业股份有限公司研究院
2. 上海海洋大学食品学院

基金项目:

国家“十二五”科技支撑计划课题(2012BAD12B08)；国家“973”计划课题(2012CB723706)；

详细信息

作者简介:
张阳旸 (1989-) , 女, 硕士研究生, 研究方向:食品微生物学与分子生物学。;

任婧 (1980-) , 女, 高级工程师, 研究方向:乳酸菌分子生物学。;

中图分类号: TS252.1
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出版历程
- 收稿日期: 2013-12-16

Study on screening and optimization of fermentation media of high protease-producing psychrotrophic bacteria

State Key Laboratory of Biotechnology Industry, Bright Dairy Research Institute
College of Food of Shanghai Ocean University

摘要

摘要: 以原料乳为对象,使用脱脂乳平板筛出三株有较强蛋白酶水解性的嗜冷菌,通过时间-酶活曲线复筛出一株高产蛋白酶的嗜冷菌Px-1。针对其产蛋白酶培养基进行了正交优化,研究了其产酶培养基的主要成分,结果表明酵母浸出粉对产酶影响显著。通过本实验最终确定了最佳培养基组成:蔗糖4%,α-乳糖4%,葡萄糖2%;酵母浸出粉0.7%,干酪素0.5%,胰蛋白胨0.3%以及0.05%的吐温80。在此条件下,Px-1产蛋白酶活为42.5451U/mL,高于优化前的测定值30.2291U/mL。
- 原料乳 /
- 嗜冷菌 /
- 蛋白酶 /
- 培养基 /
- 优化
Abstract: Three psychrotrophic strains coded Px-1, Px-3 and Px-5 which could produce protease and have obvious hydrolysis circles on defatted milk powder plate, that were isolated from raw milk. The stains were isolated again by time-enzyme activity curve. The strain Px-1 had the strongest protease activity. Composition of fermentation medium was optimized by quadratic design. The optimum fermentation medium composition was as following:4% sucrose, 4% alpha lactose, 2% glucose, 0.7% yeast extract powder, 0.5% casein, 0.3% tryptone and 0.05% tween 80. Under the optimum conditions, the protease activity of Px-1 could reach 42.5451U/mL, higher than the value before optimization 30.2291U/mL.
- raw milk /
- psychrotrophic bacteria /
- protease /
- fermentation medium /
- optimization

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