快速滴定法测定原料乳蛋白质的条件控制

张丹; 夏杨毅; 张斌斌; 尚永彪

doi:10.13386/j.issn1002-0306.2014.20.007

快速滴定法测定原料乳蛋白质的条件控制

张丹¹,
夏杨毅^1,2,3,
张斌斌¹,
尚永彪^1,2,3

1. 西南大学食品科学学院
2. 农业部农产品贮藏保鲜质量安全评估实验室(重庆)
3. 重庆市特色食品工程技术研究中心

基金项目:

(国家)公益性行业(农业)科研专项(201303144)；

详细信息

作者简介:
张丹 (1990-) , 女, 硕士研究生, 研究方向:食品微生物。;

尚永彪 (1964-) , 男, 博士, 教授, 研究方向:农产品加工。;

中图分类号: O655.2;TS252.7
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出版历程
- 收稿日期: 2014-02-10

The condition control for determination of protein in raw milk by rapid titration

College of Food Science, Southwest University
Quality and Safety Risk Assessment Laboratory of Products Preservation (Chongqing) , Ministry of Agriculture
Chongqing Special Food Programme and Technology Research Center

摘要

摘要: 目的:实验快速滴定法直接测定原料奶中蛋白质含量的可行性及掺杂物对该法的干扰程度,为原料奶验收和大批量样品检测提供快速有效的测定方法。方法:分别对快速滴定法的测定条件、操作步骤、终点判定进行反复实验,采用干燥法和凯氏定氮法对结果准确性进行验证。结果:样品取样量为1.00mL,用1.00mL 0.1mol/L盐酸控制酸度时,采用2次滴定法,消耗4.50mL 0.2%SDS,得到理想结果;与凯氏定氮法和仪器法所得结果相比,无显著差异（p>0.05）。以凯氏定氮法为标准,得到两种方法之间的数量关系,即消耗1.00mL 0.2%SDS相当于含蛋白质7.53×10-3g。一定浓度范围内的非蛋白氮掺杂物对快速滴定法测定原料奶中蛋白质的含量也无明显影响。结论:本法操作简便、结果准确,可满足原料奶验收、大批样品测定和实时掌控牛奶品质状况的要求,在奶业生产领域具有非常明显的推广应用价值。
- 快速滴定法 /
- 十二烷基硫酸钠(SDS) /
- 牛奶 /
- 蛋白质 /
- 三聚氰胺 /
- 尿素
Abstract: Objective:To test the feasibility that the direct determination of protein content in raw milk by rapid titration and the interference degree of adulteration to this method, which can provide a quick and effective way to accept the raw milk and test a large number of sample. Methods:To research the determination condition, operation steps, and the endpoint determination of rapid titration by trial and error, using the dry method and the Kjeldahl method to verify the accuracy of the results. Results:Results showed in the process of titration, 1.00 mL sample, with 1.00 mL 0.1mol/L HCl to control acidity, consumed 4.50 mL 0.2%SDS, the ideal results were obtained by second titration. Compared with Kjeldahl determination and TNM-1 instrument method, the results had no significant difference (p>0.05) . On the basis of kjeldahl determination, quantitative relationship between the two methods was obtained, namely costing 1.00 mL 0.2% SDS was equivalent to 7.53 ×10-3g protein. A certain concentration range of non-protein nitrogen dopant had no obvious effect on the determination of raw milk protein. Conclusion:The operation of this method was simple, the result was accurate, and it could meet the acceptance of raw milk, a large number of samples of milk quality measurement and real-time control the milk quality, with a very significant application value in dairy production.
- rapid titration /
- sodium dodecyl sulfate (SDS) /
- milk /
- protein /
- melamine /
- urea

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