Abstract: Male Kunming mice were randomly divided into normal, high- fat diet control group, and three levels groups treated with high ( 1.35 g /kg) , medium ( 0.90 g /kg) , and low ( 0.45 g /kg) pigments from Pu Erh tea. The changes of body weight and length of the mouse were determined every day, respectively.The levels of glucose ( Glu) , total cholesterol ( TC) , triglyceride ( TG) , high-density lipoprotein cholesterol ( HDL-C) , and low-density lipoprotein cholesterol ( LDL-C) in the serum were determined when each group was fasted for 24 h after continuous administration of 29 day, the wet weights of visceral fat of five groups were measured and the Lee's index and body fat percentage of five groups were calculated in order to observe the prevention of pigments on the weight growth of mice.The results showed that Pu Erh tea pigments had significantly effects on losing body weight, fat index, body fat percentage, and reducing Lee's index, decreasing the levels of Glu, TC, TG, LDL- C, and increasing the level of HDL- C ( p < 0.05) . Thus, Pu Erh tea pigments had the functions of preventing obesity, glucose- lowering and lipid- regulating. It could be used as a potential function drink for prevention and /or treatment of obesity, type II diabetes and dyslipidemia.