Abstract: A method was developed for measurement of alcohol content in grape wine and rice wine by ethanol biosensor.A suitable linear relationship was obtained between the concentrations of alcohol ( 2~80μL /100mL) and the correlation coefficient was 0.9997; the recovery ratio ( n = 3) were between 99.22% ~ 101.59%; the RSD% of precision and repeatability ( n = 4) were blow 1%. Alcohol content in samples evaluated by this method was compared with those evaluated by alcohol meter method, and there was no significant difference of the two methods.This method is a simple, specific, sensitive, rapid for determination of alcohol in wines, suitable for winery to control or monitor the quality of finished wine and brewing wine.