Determination of alcohol in grape wine and rice wine by ethanol biosensor
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摘要: 建立了生物传感器法测定葡萄酒和黄酒中乙醇含量的分析方法。结果表明:乙醇含量在2~80μL/100mL浓度范围内线性关系良好,相关系数为0.9997;加标回收率(n=3)在99.22%~101.59%之间;精密度和重复性(n=4)的RSD%低于1%。与国标酒精计法测定结果对比,两种方法无显著差异。该法具有无需样品前处理、特异性好、灵敏度高、测定速度快等优点,适用于企业对发酵过程控制和产品的质量检测。Abstract: A method was developed for measurement of alcohol content in grape wine and rice wine by ethanol biosensor.A suitable linear relationship was obtained between the concentrations of alcohol ( 2~80μL /100mL) and the correlation coefficient was 0.9997; the recovery ratio ( n = 3) were between 99.22% ~ 101.59%; the RSD% of precision and repeatability ( n = 4) were blow 1%. Alcohol content in samples evaluated by this method was compared with those evaluated by alcohol meter method, and there was no significant difference of the two methods.This method is a simple, specific, sensitive, rapid for determination of alcohol in wines, suitable for winery to control or monitor the quality of finished wine and brewing wine.
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Keywords:
- biosensor /
- grape wine /
- rice wine /
- alcohol
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