卵黄抗体/壳聚糖微囊的制备与体外释放性能的研究

薛海燕; 杜枘宣; 韩芳; 宋宏新

doi:10.13386/j.issn1002-0306.2014.19.003

卵黄抗体/壳聚糖微囊的制备与体外释放性能的研究

陕西科技大学生命科学与工程学院
陕西恒通果汁集团股份有限公司

基金项目:

国家自然科学基金项目(31301405)；陕西省科技统筹创新工程计划课题(2013KTZB02-02-05)；陕西省自然科学基础研究计划项目(2012JM2009)；

详细信息

作者简介:
薛海燕 (1979-) , 女, 硕士, 副教授, 研究方向:食品免疫检测技术。;

中图分类号: TQ464
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出版历程
- 收稿日期: 2014-01-21

Study on the preparation of IgY-loaded chitosan nanoparticles and release in vitro

College of Life Science and Engineering, Shaanxi University of Science and Technology
Shaanxi Heng Tong Fruit Juice Group

摘要

摘要: 研究卵黄抗体/壳聚糖缓释微囊的制备工艺。以壳聚糖为壁材,三聚磷酸钠为交联剂,采用离子交联法制备卵黄抗体（IgY）壳聚糖微囊,应用星点实验设计,以微囊对IgY的包封率和载药量为指标对壳聚糖溶液的质量浓度,壳聚糖与三聚磷酸钠质量比和IgY的初始浓度三个因素进行模型拟合,效应面分析,获得制备IgY-壳聚糖微囊的最佳工艺:壳聚糖溶液的质量浓度0.23g/100mL,壳聚糖与三聚磷酸钠质量比4∶1,IgY的质量浓度2.46mg/mL,以该条件制备的IgY-壳聚糖微囊的包封率（93.26%）和载药量（25.64%）高,与理论预测值的误差较小。IgY/壳聚糖微囊经粒度分析测得平均粒径为2.16μm,Zeta电位为26.20mV,分散度较好,体外有一定缓释作用,时间为6h,微囊化对IgY活性具有保护作用。
- IgY /
- 星点设计-效应面法优化 /
- 壳聚糖 /
- 离子交联法
Abstract: The preparation of IgY-chitosan microencapsulation and its sustained-release capacity were studied.The IgY-chitosan microencapsulation was prepared by ionic crosslinking method taking chitosan as the wall material and sodium tripolyphosphate as the crosslinking agent.Chitosan ( W/V) , chitosan /TPP ( w /w) and IgY concentration ( W/V) were recognized as main factors that affect the entrapment efficiency and the loading efficiency of the chitosan microencapsulation.Response surface analysis was used to optimize parameters of the preparation.It was found that the optimal conditions for the entrapment efficiency and the loading efficiency of chitosan microencapsulation were 0.23 g /100 mL of chitosan, 4∶1 of chitosan /TPP and 2.46 mg /mL of IgY.The actual value of the entrapment efficiency and the loading efficiency of chitosan microencapsulation were respectively 93.26% and25.64% under this conditions and the relative error was little when compared to the theoretically predicted value.The chitosan microencapsulation was characterized and showed that the mean particle size was about 2.16μm and the zeta potential was 26.20 mV analyzing by a Zetasizer. In the SGF and PBS, IgY- chitosan microencapsulation showed a good sustained- release performance for 6h. The activity of IgY in SGF was protected after microencapsulation.
- immunoglobulin Y /
- central composite design /response surface analysis /
- chitosan /
- Ionotropic Gelation method

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