汉中油菜花黄色素微波辅助提取及抗氧化活性研究

Study on the extraction and anti-oxidative activities of yellow pigment with microwave-assisted extraction from Hanzhong rapeseed flowers

摘要: 以汉中油菜花为原料,采用微波助提对所含黄色素进行提取,利用正交设计实验优化提取工艺,考察色素对食用油的抗氧化活性、对羟自由基及过氧化氢的清除能力。结果表明,油菜花黄色素的最佳提取工艺条件是:95%乙醇为提取溶剂,微波时间为30s,水浴温度为80℃,料液比为1∶40(g/mL),回流时间为15min。抗氧化实验表明,油菜花中所含黄色素具有较强的抗氧化能力,可抑制植物油氧化。在一定的浓度范围内,色素对羟自由基及过氧化氢的抗氧化活性随浓度升高而增强。

Abstract: The pigment was extracted from rapeseed flowers by microwave-assisted extraction technology. On the basis of single-factor experiments, the optimal operating conditions were obtained with orthogonal design.The optimum extraction conditions was established as follows:the concentration of ethanol 95%, extracted 30 s with microwave radiation, the reflux time was 15 min, the reaction temperature was 80℃ and the ratio of material to liquid 1∶40. The yellow pigment could inhibit the oxidant degree of the edible oil. The capacity to scavenge hydroxyl free radical and H2O2 increased as well as the concentration within a certain degree.