Abstract: To explore the influence factors impacting extraction rate of polysaccharide from Plantago asiatica L., the optimal extraction conditions using microwave-assisted extraction were optimized by single factor and orthogonal experiment, which included the solid-liquid ratio, microwave power, microwave-heating time and extraction temperature from the point of extraction rate of polysaccharide. The results showed that the effect of the factors impacting extraction rate of polysaccharide was in the order as microwave-heating time >solid-liquid ratio>extraction temperature>microwave power. The optimal conditions were as follows:the 1∶25 (g/mL) ratio of solid-liquid, microwave power was 450 W, extracting at 70℃ for 4min. The maximum amounts of polysaccharide 9.41% ±0.23% were obtained under these conditions. The method had higher rate on the extraction of polysaccharide from Plantago asiatica L. and could save the time.