HPLC-DAD法同时测定金银花中7种多酚类物质

游玉明; 陈泽雄; 张美霞; 冉烈

doi:10.13386/j.issn1002-0306.2014.16.003

HPLC-DAD法同时测定金银花中7种多酚类物质

重庆文理学院林学与生命科学学院

基金项目:

国家自然科学基金项目(31200512)；重庆市教委科技项目(KJ1401101)；

详细信息

作者简介:
游玉明 (1983-) , 男, 在读博士, 讲师, 研究方向:食品化学与营养学。;

中图分类号: O657.72;R284.1
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出版历程
- 收稿日期: 2013-10-11

Simultaneous determination of seven phenolic compounds in Lonicera japonica by HPLC-DAD

College of Forestry and Life Science, Chongqing University of Arts and Sciences

摘要

摘要: 建立了高效液相色谱-二极管阵列法（HPLC-DAD）同时测定金银花中绿原酸、咖啡酸、阿魏酸、香豆酸、肉桂酸、芦丁和木犀草苷的方法。采用岛津Shim-packVP-ODS C18（250mm×4.6mm,5μm）色谱柱,2%甲酸-2%甲酸甲醇溶液为流动相梯度洗脱,柱温35℃,流速0.7mL/min。结果表明:绿原酸、咖啡酸、阿魏酸、香豆酸、肉桂酸、芦丁和木犀草苷分别在1.04⁵20、1.02⁵10、1.59⁵30、1.26⁶30、1.89⁵60、1.62⁵40、0.29⁵8mg/L范围内线性关系良好（r值范围为0.9964⁰.9999）,检出限（RSN=3）为0.001⁰.078mg/L,精密度RSD小于3.27%,平均回收率为93.42%¹02.53%。本方法操作简单,灵敏度高、重现性好,可用于金银花中7种多酚类物质的定量分析和质量监控。
- 金银花 /
- 高效液相色谱-二极管阵列 /
- 多酚类物质
Abstract: A high performance liquid chromatography with diode array detection (HPLC-DAD) method was developed for the simultaneous determination of seven phenolic compounds including chlorogenic acid, caffeic acid, ferulic acid, p-coumalic acid, trans-cinnamic acid, rutin and luteolin-7-O-glucoside in Lonicera japonica.The samples were separated with a ODS-C18column (250mm×4.6mm, 5μm Shimadzu Corporation) by gradient elution using 2% formic acid in water and 2% formic acid in methanol as the mobile phase at a flow rate of0.7mL/min in 35℃. The standard curve revealed a good linear relationship (r=0.9964~0.9999) over a range of1.04~520mg/L for chlorogenic acid, 1.02~510mg/L for caffeic acid, 1.59~530mg/L for ferulic acid, 1.26~630mg/L for p-coumalic acid, 1.89~560mg/L for trans-cinnamic acid, 1.62~540mg/L for rutin and 0.29~58mg/L for luteolin-7-O-glucoside respectively. The limits of detection were ranged from 0.001 to 0.078mg/L (RSN=3) . The average recovery rates were 93.42%~102.53% and the precisions <3.27%. This method was simple, sensitive with good reproducibility and could be used for qualitative evaluation and quality control of seven phenolic compounds in Lonicera japonica.
- Lonicera japonica /
- HPLC-DAD /
- phenolic compounds

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参考文献(27)

[1]	关炳峰, 谭军, 周志娣.金银花提取物的抗氧化作用与其绿原酸含量的相关性研究[J].食品工业科技, 2007, 28 (10) :127-129.
[2]	Choi C W, Jung H A, Kang S S, et al.Antioxidant Constituents and a New Triterpenoid Glycoside from Flos Lonicera[J].Arch Pharmacal Res, 2007, 30 (1) :1-7.
[3]	王芳, 蒋跃平, 王晓良, 等.金银花的化学成分研究[J].中国中药杂志, 2013, 38 (9) :1378-1385.
[4]	Liao Yanyan, Dong Sijun, Kilama R, et al.Flos Lonicerae Extracts and Chlorogenic Acid Protect Human Umbilical Vein Endothelial Cells from the Toxic Damage of Perfluorooctane Sulphonate[J].J Inflammation, 2013, 36 (3) :767-779.
[5]	Chen Weicheng, Liou S S, Tzeng T F, et al.Wound Repair and Anti-inflammatory Potential of Lonicera japonica in Excision Wound-induced Rats[J].Complementary&Alternative Medicine, 2012, 12:226-234.
[6]	Lee H, Lee G, Yoon M S, et al.Effect of Fermented Lonicera japonica on LPS-induced Acute Lung Inflammation[J].Orient Pharm Exp Med, 2011, 11 (4) :287-292.
[7]	Teng Yang, Sun Changhai, Li Guangzhi, et al.Protective Effects of Flos lonicera Extract on Acute Liver Injury by Dimethylnitrosamine-induced in Rats[J].J Nat Med, 2010, 64 (3) :288-294.
[8]	陈雅文.浅谈金银花的化学成分、药理作用和实用价值[J].中国民间疗法, 2013, 21 (5) :70-71.
[9]	Narang J, Chawla S, Chuauhan N, et al.Construction of an amperometric polyphenol biosensor based on PVA membrane[J].Food Measure, 2013, 7 (1) :22-28.
[10]	国家药典委员会.中国药典[S].一部.北京:中国医药科技出版社, 2010:205-206.
[11]	陈奇, 邓雁如, 夏娜.金银花多酚类物质的纯化研究[J].药物评价, 2012, 35 (3) :185-189.
[12]	肖卓炳, 郭瑞轲, 郭满满, 等.金银花中绿原酸超声微波双辅助提取工艺优化[J].食品科学, 2012, 33 (22) :111-114.
[13]	Zhou Yuanzhen, Liu Xiaoxing, Zheng Xiaohui, et al.Simultaneous Determination of Quercetin and Luteolin in Dried Flowers by Multivariate HPLC-ECD Calibration[J].Chromatographia, 2007, 66 (7/8) :635-637.
[14]	Ren Jing, Jiang Xuehua, Li Chenrui, et al.Investigation on the Absorption Kinetics of Chlorogenic Acid in Rats by HPLC[J].Archives of Pharmacal Research, 2007, 30 (7) :911-916.
[15]	Zhao Y K, Cao Q E, Liu H T, et al.Determination of Baicalin, Chlorogenic Acid and Caffeic Acid in Traditional Chinese Medicinal Preparations by Capillary Zone Electrophoresis[J].Chromatographia, 2000, 51 (7/8) :483-486.
[16]	颜流水, 温振东, 黄智敏, 等.毛细管电泳法测定金银花中绿原酸、芦丁和槲皮素[J].药物分析杂志, 2007, 27 (3) :367-370.
[17]	Qi Lianwen, Chen Chunyun, Li Ping.Structural Characterization and Identification of Iridoid Glycosides, Saponins, Phenolic Acids and Flavonoids in Flos Lonicerae Japonicae by a Fast Liquid Chromatography Method with Diodearray Detection and Time-of-flight Mass Spectrometry[J].Rapid Commun Mass Spectrom, 2009, 23 (19) :3227-3242.
[18]	Luo Yongdong, Wu Shuisheng, Li Xiaoyan, et al.LC-ESIMS-MS Determination of Rat Plasma Protein Binding of Major Flavonoids of Flos Lonicerae Japonicae by Centrifugal Ultrafiltration[J].Chromatographia, 2012, 72 (1) :71-77.
[19]	胥秀英, 郑一敏, 傅善权, 等.高效液相色谱法测定金银花中木犀草素的含量[J].食品科学, 2006, 27 (5) :221-222.
[20]	王艳艳, 徐晓玉, 邓君, 等.HPLC法同时测定灰毡毛忍冬中绿原酸与木犀草苷的含量[J].中药材, 2009, 32 (11) :1705-1707.
[21]	王丽婷, 杨敏丽.高效液相色谱法同时测定金银花及叶中60的黄酮类物质[J].时珍国医国药, 2007, 18 (8) :1850-1851.
[22]	辛华, 丰杰, 程若敏, 等.HPLC测定不同产地金银花中绿原酸和木犀草苷[J].中国实验方剂学杂志, 2011, 17 (2) :60-63.
[23]	Yuan Yan, Song Lipu, Li Guiming, et al.Genetic Variation and Metabolic Pathway Intricacy Govern the Active Compound Content and Quality of the Chinese Medicinal Plant Lonicera Japonica Thunb[J].Genomics, 2012, 13:195-211.
[24]	Tarola A M, Velde F V, Salvagni L, et al.Determination of Phenolic Compounds in Stawberries (Fragaria ananassa Duch) by High Performance Liquid Chromatography with Diode Array Detection[J].Food Anal Methods, 2013, 6 (1) :227-237.
[25]	Tian Su, Nakamura K, Cut Tong, et al.High-performance Liquid Chromatography Determination of Phenolic Compounds in rice[J].J Chromatography A, 2005, 1063 (1/2) :121-128.
[26]	马亚琴, 叶兴乾, 吴厚玖, 等.超声处理对琯溪蜜柚皮中多组分酚酸稳定性的影响[J].食品科学, 2013, 34 (19) :27-31.
[27]	刘伟, 郑振佳, 周洁, 等.良种金银花不同采收期发育程度与品质比较研究[J].山东科学, 2012, 25 (2) :35-38.