Research advance in the strain breeding and gene modification of α-Amylase
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摘要: α-淀粉酶是重要的工业用酶制剂之一,能在高温、低pH范围内稳定发挥作用的α-淀粉酶则具有更加广阔的应用前景。本文主要从基因改造与菌种选育两方面阐述了高温α-淀粉酶、酸性α-淀粉酶以及高温酸性α-淀粉酶的研究与开发进展。Abstract: α-amylase is a kind of the most important enzymes utilized in industry.Moreover, the α-amylases which can keep stable and active under acid and hyperthermal conditions are used more widely. In terms of gene modification and strain screening, the research and development progress of thermostable α-amylase, acid stableα-amylase and thermostable, acidophilic α-amylase were reviewed.
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Keywords:
- α-amylase /
- gene modification /
- strain breeding
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[1] Saeeda B, Shah A, Afsheen A, et al.Purification and characterization of novelα-amylase from Bacillus subtilis KIBGE HAS[J].The American Association of Pharmaceutical Scientists, 2011, 12 (1) :255-261.
[2] Wei Y, Wang X, Liang J, et al.Identification of a halophilic a-amylase gene from Escherichia coli JM109 and characterization of the recombinant enzyme[J].Biotechnology Letters, 2013, 35 (7) :1061-1065.
[3] Prakash O, Jaiswal N.α-Amylase:an ideal representative of thermostable enzymes[J].Applied Biochemistry and Biotechnology, 2010, 160 (8) :2401-2414.
[4] Crabb W D, Mitchinson C.Enzymes involved in the processing of starch to sugars[J].Trends in Biotechnology, 1997, 15 (9) :349-352.
[5] Liu Y, Shen W, Shi G Y, et al.Role of the calcium-binding residues Asp231, Asp233, and Asp438 in alpha-amylase of Bacillus amyloliquefaciens as revealed by mutational analysis[J].Current Microbiology, 2010, 60 (3) :162-166.
[6] Paula M S, Perola O M.Application of microbialα-amylase in industry-a review[J].Brizilian Journal of Microbilogy, 2010, 41 (4) :850-861.
[7] Mischa M, Nathalie D, Robert H, et al.Activation of Bacillus licheniformisα-amylase through a disorder→order transition of the substrate-binding site mediated by a calcium-sodiumcalcium metal triad[J].Structure, 2013, 21 (9) :1477-1718.
[8] 王楠, 马荣山.耐高温α-淀粉酶的研究进展[J].中国食品添加剂, 2007, 4 (9) :90-92. [9] Declerck N, Machius M, Wiegand G, et al.Probing structural determinants specifying high thermostability in Bacillus licheniformisα-Amylase[J].Journal of Molecular Biology, 2000, 301 (4) :1041-1057.
[10] Declerck N, Machius M, Chambert R, et al.Hyperthermostable mutants of Bacillus licheniformis alphaamylase:thermodynamic studies and structural interpretation[J].Protein Engineering Design and Selection, 1997, 10 (5) :541-549.
[11] Antrim R L, Barnett C C, Mitchinson C, et al.Oxidatively stable a-amylase:EP, 0689589[P].2009-2-9.
[12] Mitchinson C.Mutantα-amylase:US, 5736499[P].1998-4-7.
[13] Chi M C, Chen Y H, Wu T J, et al.Engineering of a truncatedα-amylase of Bacillus sp.strain TS-23 for the simultaneous improvement of thermal and oxidative stabilities[J].Journal of Bioscience and Bioengineering, 2010, 109 (6) :531-538.
[14] Tomazic S J, Klibanov A M.Mechanisms of irreversible thermal inactivation of Bacillus alpha-amylases[J].The Journal of Biological Chemistry, 1988, 263 (5) :3086-3091.
[15] Machius M, Declerck N, Huber R, et al.Kinetic stabilization of Bacillus licheniformisα-amylase through introduction of hydrophobic residues at the Surface[J].The Journal of Biological Chemistry, 2003, 278 (13) :11546-11553.
[16] Mauno V, Taina P, Antti L, et al.C-terminal truncations of a thermostable Bacillus stearothermophilusα-amylase[J].Protein Engineering Design and Selection, 1994, 7 (10) :1255-1259.
[17] Kohji O, Takashi K, Hiroki K, et al.Characteristics of two forms ofα-Amylases and structural implication[J].Applied and Environmental Microbiology, 1999, 65 (10) :4652-4658.
[18] 王磊, 周新宇, 唐上华, 等.耐高温α-淀粉酶基因在枯草杆菌染色体上的整合、扩增及表达的研究[J].工业微生物, 1998, 28 (1) :7-11. [19] 董晨, 曹娟, 张迹, 等.耐高温α-淀粉酶基因在枯草芽孢杆菌中的高效表达[J].应用与环境生物学报, 2008, 14 (4) :534-538. [20] Andrew S, Richard B, Anthony G D, et al.Protein engineering ofα-amylase for low pH performance[J].Current Opinion in Biotechnology, 1999, 10 (4) :349-352.
[21] Liu Y H, Hu B, Xu Y J, et al.Improvement of the acid stability of Bacillus licheniformis alpha-amylase by error-prone PCR[J].Journal of Applied Microbiology, 2012, 113 (3) :541-549.
[22] Liu X D, Xu Y.A novel raw starch digestingα-amylase from a newly isolated Bacillus sp.YX-1:Purification and characterization[J].Bioresource Technology, 2008, 99 (1) :4314-4320.
[23] Liu X D, Xu Y.A novel raw starch digestingα-amylase from a newly isolated Bacillus sp.YX-1:Purification and characterization[J].Bioresource Technology, 2008, 99 (1) :4314-4320.
[24] Shen H P, Mo X Y, Chen X, et al.Purification and enzymatic identification of an acid stable and thermostableα-amylase from Rhizopus microsporus[J].Journal of The Institute of Brewing, 2012, 118 (3) :309-314.
[25] Ahmad A, Jamshidkhan C, Milad L.A novel thermostable, acidophilicα-amylase from a new thermophilic“Bacillus sp.Ferdowsicous”isolated from Ferdows hot mineral spring in Iran:Purification and biochemical characterization[J].International Journal of Biological Macromolecules, 2010, 46 (3) :289-297.
[26] Heng C, Chen Z J, Du L X, et al.Expression and secretion of an acid-stableα-amylase gene in Bacillus Subtilis by sacB promoter and signal peptide[J].Biotechnology Letters, 2005, 27 (21) :1731-1737.
[27] Mehta D, Satyananrayana T.Biochemical and molecular characterization of recombinant acidic and thermostable rawstarch hydrolyzingα-amylase from an extreme thermophile Geobacillus thermoleovorans[J].Journal of Molecular Catalysis B:Enzymatic, 2013, 85 (3) :229-238.
[28] 曾庆梅, 魏春燕, 靳靖, 等.黑曲霉酸性α-淀粉酶基因的克隆及其在毕赤酵母中的表达[J].食品科学, 2011, 32 (17) :219-224. [29] 袁铁铮, 姚斌卜, 罗会颖, 等.来源于酸热脂环酸杆菌的嗜酸性α-淀粉酶的表达研究[J].生物工程学报, 2005, 21 (1) :78-83. [30] 崔锦, 柯涛, 马向东.α-淀粉酶基因在毕赤酵母中的表达及酶学性质研究[J].河南农业大学学报, 2006, 40 (3) :311-314. [31] Archana S.High maltose-forming, Ca2+-independent and acid stableα-amylase from a novel acidophilic bacterium, Bacillus acidicola[J].Biotechnology Letters, 2010, 32 (10) :1503-1507.
[32] 戚薇王海燕, 王建玲, 等.氮离子注入选育耐酸性α-淀粉酶产生菌诱变效应的研究[J].天津科技大学学报, 2007, 22 (3) :6-9. [33] 陈远钊, 罗俊成, 胡佳, 等.酸性α-淀粉酶菌株的诱变及酶的性质研究[J].中国酿造, 2007, 166 (1) :10-13. [34] Nedwin G E, Sharma V, Shetty J K, et al.Alpha-amylase blend for starch processing and method of use thereof:US:20120171731A1[P].2012-7-5.
[35] Shafaq A H, Syed A, Atiya A.Purification and biochemical characterization of a Ca2+-independent, thermostable and acidophilicα-amylase from Bacillus sp.RM16[J].African Journal of Biotechnology, 2011, 10 (32) :6082-6089.
[36] Richardson T H, Tan X, Frey G, et al.A Novel, High performance enzyme for starch liquefaction:discovery and optimization of a low pH, thermostableα-amylase[J].The Journal of Biological Chemistry, 2002, 277 (29) :26501-26507.
[37] 郭建强, 李运敏, 岳丽丽, 等.超耐热酸性α-淀粉酶基因的克隆及其在酵母细胞中的表达[J].生物工程学报, 2006, 22 (2) :238-242. [38] 郝帅, 郭建强, 李运敏, 等.超耐热酸性α-淀粉酶基因在多型汉逊酵母中表达及与在毕赤酵母中表达的比较研究[J].菌物学报, 2006, 25 (4) :573-578. [39] Devi L S, Khaund P, Joshi S R.Thermostableα-amylase from natural variants of Bacillus spp.prevalent in eastern Himalayan Range[J].African Journal of Microbiology Research, 2010, 23 (4) :2534-2542.
[40] Guan Q, Guo X, Han T, et al.Cloning, purification and biochemical characterisation of an organic solvent-, detergent-, and thermo-stable amylopullulanase from Thermococcus kodakarensis KOD1[J].Process Biochemistry, 2013, 48 (5) :878-884.
[41] Ying Q, Zhang C, Guo F, et al.Secreted Expression of a Hyperthermophilicα-Amylase Gene from Thermococcus sp.HJ21in Bacillus subtilis[J].Journal of Molecular Microbiology and Biotechnology, 2012, 22 (6) :392-398.
[42] Yang S J, Min B C, Kim Y W, et al.Changes in the catalytic properties of Pyrococcus furiosus thermostable amylase by mutagenesis of the substrate binding sites[J].Applied and Environmental Microbiology, 2007, 73 (17) :5607-5612.
[43] Carol D L.Potential for industrial products from the halophilic Archaea[J].Journal of Industrial Microbiology and Biotechnology, 2011, 38 (10) :1635-1647.
[44] Sharma A, Satyanarayana T.Microbial acid-stableα-amylases:Characteristics, genetic engineering and applications[J].Process Biochemistry, 2013, 48 (2) :201-211.
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