液相色谱-质谱法测定食用植物油中缩水甘油酯的含量

刘京; 张晖; 王瑛瑶; 段章群; 王立

doi:10.13386/j.issn1002-0306.2014.15.059

液相色谱-质谱法测定食用植物油中缩水甘油酯的含量

刘京^1,2,
张晖¹,
王瑛瑶²,
段章群²,
王立¹

1. 江南大学食品学院
2. 国家粮食局科学研究院

基金项目:

2013年粮食公益性行业科研专项(201313011-7)；国家863计划课题(2013AA102104)；

详细信息

作者简介:
刘京 (1988-) , 女, 硕士研究生, 研究方向:粮油检测。;

中图分类号: TS227;O657.63
计量
- 文章访问数: 143
- HTML全文浏览量: 19
- PDF下载量: 329
出版历程
- 收稿日期: 2013-12-11

Determination of glycidyl esters in edible vegetable oils by liquid chromatography-mass spectrometry

College of Food Science, Jiangnan University
Academy of State Administration of Grain

摘要

摘要: 采用液相色谱-质谱法检测食用油脂中缩水甘油酯的含量,以AOCS方法为基础,优化了前处理条件和流动相梯度洗脱程序。方法的缩水甘油酯回收率为92.42%¹09.20%,相对标准偏差RSD为4.01%⁷.52%,五种缩水甘油酯的最低检出限在9³0μg/kg之间。本方法灵敏度高,重复性好,能够满足实际工作中油脂中缩水甘油酯含量分析的需要。
- 缩水甘油酯 /
- 液相色谱-质谱 /
- 固相萃取 /
- 食用植物油
Abstract: In this paper, the GEs were detected by liquid chromatography- mass spectrometry. This method had been optimized the pretreatment conditions and the mobile phase gradient elution program based on the AOCS.The recovery values of GEs ranged from 92.42% to 109.20%, meanwhile the RSD was 4.01% to 7.52%. The minimum detection limit of five GEs ranged from 9μg /kg to 30μg /kg.This method had high sensitivity and good repeatability.This method can meet the need of content analysis of glycidyl ester in practical work.
- GEs /
- liquid chromatography-mass spectrometry /
- solid phase extraction /
- edible vegetable oils

HTML全文

参考文献(8)

[1]	陈华勇, 唐小红, 王永华, 等.GC-MS定性分析食用油中的缩水甘油脂有害物[J].油脂安全, 2012, 37 (3) :66-69.
[2]	Joint FAO/WHO Expert committee on food additives[C]//Summary of the Fifty-seventh Meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA) .Rome, 2001:5-14, 20-24.
[3]	DGF Standard Methods C-Ⅲ18 (09) .Ester-bound 3-chloropropane-1, 2-diol (3-MCPD esters) and glycidol (glycidyl esters) .Determination in fats and oils by GC-MS[S].
[4]	Ermacora A, Hrncirik K.A novel method for simultaneous monitoring of 2-MCPD, 3-MCPD and glycidyl esters in oils and fats[J].J Am Oil Chem Soc, 2012, DOI: 10.1007/s11746-012-2132-9.
[5]	Haines T D, Adlaf K J, Pierceall R M, et al.Direct Determination of MCPD Fatty Acid Esters and Glycidyl Fatty Acid Esters in Vegetable Oils by LC-TOFMS[J].J Am Oil Chem Soc, 2011 (88) :1-14.
[6]	Hori K, Koriyama N, Omori H, et al.Simultaneous determination of 3-MCPD fatty acid esters and glycidol fatty acid esters in edible oils using liquid chromatography time-of-flight mass spectrometry[J].LWT-Food Science and Technology, 2012:1-5.
[7]	王力清, 陈洪涛, 廖继承, 等.同位素稀释GC-MS/MS方法测定油脂中3-MCPD脂肪酸酯和缩水甘油脂肪酸酯的总量[J].油脂工程, 2011, 8:61-63.
[8]	Joint AOCS/JOCS Recommended Practice Cd 28-10.This method is used for the determination of glycidyl (glycidol) fatty acid esters (GEs) in edible oils using double solid-phase extraction (SPE) and liquid chromatography-mass spectrometry (LC-MS) using HPLC.2011.