液相色谱-质谱法测定食用植物油中缩水甘油酯的含量

Determination of glycidyl esters in edible vegetable oils by liquid chromatography-mass spectrometry

摘要: 采用液相色谱-质谱法检测食用油脂中缩水甘油酯的含量,以AOCS方法为基础,优化了前处理条件和流动相梯度洗脱程序。方法的缩水甘油酯回收率为92.42%¹09.20%,相对标准偏差RSD为4.01%⁷.52%,五种缩水甘油酯的最低检出限在9³0μg/kg之间。本方法灵敏度高,重复性好,能够满足实际工作中油脂中缩水甘油酯含量分析的需要。

Abstract: In this paper, the GEs were detected by liquid chromatography- mass spectrometry. This method had been optimized the pretreatment conditions and the mobile phase gradient elution program based on the AOCS.The recovery values of GEs ranged from 92.42% to 109.20%, meanwhile the RSD was 4.01% to 7.52%. The minimum detection limit of five GEs ranged from 9μg /kg to 30μg /kg.This method had high sensitivity and good repeatability.This method can meet the need of content analysis of glycidyl ester in practical work.