Research of the optimization of extraction process of Euphausia pacifica phospholipid and analysis of fatty acids composition
-
摘要: 本研究以太平洋磷虾为原料,着重探讨了乙醇浓度、液固比、提取温度、提取时间对磷虾磷脂得率及纯度的影响。结合单因素实验与正交实验分析,结果表明:最佳工艺条件为乙醇浓度95%、液固比6mL/g,提取时间4h,提取温度40℃,在此工艺条件下粗磷脂得率为17.58%,纯度为46.09%。再经4℃丙酮沉降后,磷脂纯度为78.85%。产物通过气相色谱分析,不饱和脂肪酸占52.46%,在多不饱和脂肪酸中EPA和DHA含量之和最高,其占总脂肪酸含量的24.51%。Abstract: The effects of ethanol concentration, the ratios of solvent to krill sample, extraction temperature and extraction time on the yield and purity of phospholipids extracted from Euphausia pacifica was investigated.Judging from the results of single factor experiment and orthogonal experiment, the optimal processing conditions were as follows: 95% ethanol, 6 to 1 ratio of solvent to krill sample, a 4h extraction time and a 40℃ extraction temperature.Under such conditions, the reasonable yield of 17.58% and purity of 46.09% were obtained.The purity reached 78.85% with a further purification by acetone at 4℃.The fatty acids of the further purified phospholipids were then analysed by GC.Results showed that the unsaturated fatty acids were 52.46%, the total percentage of EPA and DHA were 24.51% among all the fatty acids analysed.
-
Keywords:
- Euphausia pacifica /
- phospholipid /
- fatty acid analysis
-
[1] 江尧森, 王家林, 殷邦忠.太平洋磷虾虾汁加工的研究[J].食品科学, 1986, 7 (1) :39-45. [2] 王兰, 冯晓梅, 吕晴.磷虾肌肉营养成分的分析和评价[J].中国海洋大学学报, 2013, 4 (7) :51-55. [3] 崔秀明, 汪之和, 施文正.南极磷虾粗虾油提取工艺优化[J].食品科学, 2011, 32 (24) :126-129. [4] Nehari A A, Chun B S.Characterization of purified phospholipids from krill (Euphausi superba) residues deoiled by supercritical carbon dioxide[J].Korean J Chem Eng, 2012, 29 (7) :919-923.
[5] 李璇, 郑建仙.脂肪与心血管疾病相互关系最新进展及对食品工业的指导意义[J].食品与发酵工业, 1998, 24 (1) :74-79. [6] 郝颖, 汪之和.EPA、DHA的营养功能及其产品安全性分析[J].现代食品科技, 2006, 22 (3) :180-183. [7] 刘坤, 王兰, 薛长湖, 等.太平洋磷虾油的亚临界R134a萃取工艺及脂肪酸组成分析[J].食品科学, 2013, 34 (14) :96-99. [8] 梁萌青, 王士稳, 韩庆炜, 等.海水养殖和低盐养殖凡纳滨对虾脂肪酸分析比较[J].渔业科学进展, 2009, 30 (1) :87-91. [9] Hixson C, Stefanie M, Olsen R E, et al.Lipid and fatty acid digestibility in Calanus copepod and krill oil by Atlantic halibut (Hippoglossus hippoglossus L.) [J].Aquaculture, 2011, 313:115-116.
[10] Saito H, Kotani Y, Keriko J M, et al.High levels of n-3polyunsaturated fatty acids in Euphusia pacifica and its role as a source of docosahexaenoic and icosapentaecoic acids for higher trophic levels[J].Mar Chem, 2002, 78:9-28.
[11] 王兰, 岳荣岩, 卢杰, 等.太平洋磷虾脂肪酸的气相色谱-质谱法分析[J].现代食品科技, 2012, 28 (10) :1408-1410. [12] 楼乔明, 王玉明, 刘小芳, 等.南极磷虾脂肪酸组成及多不饱和脂肪酸质谱特征分析[J].中国水产科学, 2011, 18 (2) :1258-1261. [13] Song J H, Inoue Y, Miyazawa T.Oxidative stability of docosahexaenoic acid-containing oils in the form of phospholipids, triacylglycerols and ethyl esters[J].Biosci Biotech Biochem, 1997, 61:2085-2088.
[14] Coste T C, Gerbi A, Vague P, et al.Neuroprotective effect of docosahexaenoic acid-enriched phospholipids in experimental diabetic neuropathy[J].Diabetes, 2003, 52 (10) :2579-2585.
[15] Phleger C F, Nelson M M, Mooney B D, et al.Interannual and between species comparison of the lipids fatty acids and sterols of Antarctic krill from the US AMLR Elephant Island survey area[J].Comparative Biochemistry and Physiology Part B, 2002, 131 (4) :733-745.
-
期刊类型引用(5)
1. 张练，王茂娟，谢红，周荣，江帆，雷贤英. 迷迭香酸对脓毒症急性肾损伤大鼠NF-κB和p38 MAPK信号通路的影响. 现代免疫学. 2024(03): 221-226+233 . 
百度学术
2. 郭誉嵘，王丹，李河，武瑞杰，崔小芳，许瑞清，李会珍，张志军. 紫苏叶提取物对美拉德体系晚期糖基化终末产物生成的抑制作用研究. 食品安全质量检测学报. 2023(16): 143-151 . 百度学术
3. 郭笑，林睿，叶思萍，杨莹莹，侯志东，盛瑜，安丽萍. 蛹虫草活性成分的提取分离及体外抗氧化作用研究. 中国药学杂志. 2022(05): 357-362 . 百度学术
4. 任志清，李会珍，张志军，N.Vasudeva Reddy，赵亚娜，李河. 不同品种紫苏叶迷迭香酸的提取及其生物活性. 现代食品科技. 2021(01): 92-100 . 百度学术
5. 蹇黎，杨婧，余丹凤，秦小军. V_C对盐胁迫下紫苏种子萌发的影响. 安徽农业科学. 2021(18): 52-54 . 百度学术
其他类型引用(3)
计量
- 文章访问数: 137
- HTML全文浏览量: 16
- PDF下载量: 265
- 被引次数: 8
下载:
