高压蒸汽提取啤酒废酵母还原型谷胱甘肽的工艺优化

冷非凡; 王晓力; 王永刚; 任海伟; 庄岩; 朱新强; 孙尚琛

doi:10.13386/j.issn1002-0306.2014.15.040

高压蒸汽提取啤酒废酵母还原型谷胱甘肽的工艺优化

1. 兰州理工大学生命科学与工程学院
2. 中国农业科学院兰州畜牧与兽药研究所

基金项目:

农业行业科研专项:饲草饲料资源开发利用技术研究与示范(201203042)；甘肃省自然基金(1212RJYA008,1308RJZA287,2013GS09588)；

详细信息

作者简介:
冷非凡 (1981-) , 男, 副教授, 研究方向:微生物学。;

中图分类号: TS261.9
计量
- 文章访问数: 151
- HTML全文浏览量: 15
- PDF下载量: 119
出版历程
- 收稿日期: 2013-08-06

Optimization of extraction parameters for Glutathione from beer waste brewing yeast treated by high-pressure steam (HPS)

School of Life Science and Engineering, Lanzhou University of Technology
Lanzhou Institute of Animal Husbandry and Veterinary Medicine, China Academy of Agricultural Sciences

摘要

摘要: 为了得到高压蒸汽提取废酵母中还原型谷胱甘肽的最佳工艺条件,利用Box-Behnken的中心组合设计及响应面法（RSM）探讨了表压、提取时间、液料比和提取次数四因素的优化组合。通过建立二次回归模型,确定其最佳提取工艺条件为:表压1.1MPa,提取时间15min,液料比10∶1,提取次数1次,在此条件下得率最大为4.63mg/g。结果表明高压蒸汽提取技术是提高啤酒废酵母还原型谷胱甘肽得率的有效途径之一。
- 高压蒸汽 /
- 还原型谷胱甘肽 /
- 啤酒废酵母 /
- 提取 /
- 响应面
Abstract: To establish the extraction technology of Glutathione from beer waste brewing yeast by high- pressure steam, the optimization was applied to discuss some factors including the pressure, extracting time, the ratio of solid to liquid and extracting times with Box-Behnken center-united design and the method of response surface analysis ( RSM) . Through establishing the second regression model, the optimal parameters were conformed as follows: the pressure 1.1MPa, extracting time 15 min, the ratio of solid to liquid 10∶1 and extracting times 1.Under this conditions, the yield of GSH was 4.63 mg /g. These results suggested that the high- pressure steam extraction technology was one of the effective ways to improve the yield of reduced glutathione.
- high-pressure steam /
- reduced glutathione /
- beer waste brewing yeast /
- extraction /
- response surface methodology

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