响应面法优化小丛红景天多糖超声提取工艺研究

王莉; 赵桦; 马英丽; 王星

doi:10.13386/j.issn1002-0306.2014.14.061

响应面法优化小丛红景天多糖超声提取工艺研究

陕西理工学院生物科学与工程学院

基金项目:

陕西省教育厅专项科研计划项目(12JK0842)；陕西省重点学科专项建设经费资助(2013)；

详细信息

作者简介:
王莉 (1987-) , 女, 在读硕士研究生, 研究方向:植物资源开发利用研究。;

中图分类号: TQ461
计量
- 文章访问数: 0183
- HTML全文浏览量: 029
- PDF下载量: 0374
出版历程
- 收稿日期: 2013-09-27

Optimization of ultrasonic-assisted extraction of polysaccharides from Rhodiola dumulosa (Franch.) S.H.F. by response surface analysis

School of Biological Science and Engineering, Shaanxi University of Technology

摘要

摘要: 为优化小丛红景天药材中水溶性多糖超声提取工艺,在单因素实验的基础上,利用响应面分析方法建立了小丛红景天多糖超声提取的二次响应曲面方程。实验证实提取温度、超声功率、提取时间和料液比对小丛红景天多糖提取有不同的影响。结果表明,在实验范围内通过方差分析可知各因素对小丛红景天多糖提取率影响的大小依次为超声提取温度、超声功率、料液比和超声提取时间。小丛红景天多糖超声提取工艺的最佳条件为:提取温度69℃、超声功率240W、料液比1∶30(g/mL),提取时间39min,在此条件下小丛红景天多糖提取率可达4.305%,与模型预测值高度相符。
- 小丛红景天 /
- 多糖 /
- 超声提取 /
- 响应面分析法
Abstract: In order to optimize the conditions of ultrasonic-assisted extraction method of polysaccharides from Rhodiola dumulosa (Franch.) S.H.F., on the base of single-factor experiment, an equation for the ultrasonic wave extraction was built. The experimence showed that extraction temperature, ultrasonic power, extraction time and liquid-solid ratio had different effects on the extraction yield of polysaccharides. The result indicated that within tested level ranges, the importance of four process parameters affecting extraction yield of polysaccharides order was ultrasonic treatment temperature, ultrasonic power, solid-liquid ratio and ultrasonic treatment time. The optimum conditions of the ultrasonic wave extraction of R. dumulosa polysaccharides were as follows:temperature 69℃, extraction ultrasonic power 240W, solid-liquid ratio 1∶30 (g/mL) and extraction time 39min, the extraction rate of R. dumulosa polysaccharides could be up to 4.305%, exhibiting a good agreement with the predicted values.
- Rhodiola dumulosa (Franch.) S.H.F. /
- polysaccharides /
- ultrasonic extraction /
- response surface methodology

HTML全文

参考文献(18)

[1]	中科院中国植物志编委会.中国植物志, 34卷[M].第一分册.北京:科学出版社, 1984, 159-171.
[2]	吴振海, 傅青, 陈书文.陕西民间“七药”种类整理[J].中药材, 1990, 13 (5) :14-16.
[3]	郭增军, 卜筱茜, 王军宪, 等.陕西“七药”植物资源及研究概要[J].中国民族民间医药杂志, 2006, 79:79-81.
[4]	黄宏卿, 曹丽华, 张陆兵, 等.红景天药理活性研究进展[J].中国实用医药, 2006, 1 (1) :57-65.
[5]	罗定强, 赵翔宇, 王军宪.小丛红景天化学成分的研究 (I) [J].中药材, 2005, 28 (2) :98-99.
[6]	王军宪, 罗定强, 赵翔宇.小丛红景天化学成分的研究 (Ⅱ) [J].中药材, 2006, 29 (4) :335-336.
[7]	王莉, 赵桦, 徐皓.小丛红景天多糖含量测定及生物学活性研究[J].天然产物研究与开发, 2013, 25 (11) :1536-1540.
[8]	王慧娜, 赵桦.响应面法优化开口箭多糖超声提取工艺研究[J].食品工业科技, 2012, 33 (13) :187-192.
[9]	陈慧, 李雪虎, 陆锡虎, 等.响应面超声辅助提取优化蛇床子素工艺的研究[J].天然产物开发与研究, 2013, 25 (6) :834-840.
[10]	张秋龙, 梁永欣, 李文聪, 等.响应面法优化细果角茴香中总黄酮的超声提取条件[J].天然产物开发与研究, 2013, 25 (6) :841-845.
[11]	林志娟, 陈永, 尤丽彤, 等.响应面法优化超声辅助提取太子参多糖工艺研究[J].天然产物开发与研究, 2013, 25 (6) :846-850.
[12]	Box GP, Behnken DW.Some new three level design for the study of quantitative variables[J].Techno Metrics, 1960, 2:456-475.
[13]	Lee WC, Yusof S, Hamid NSA, et al.Optimizing conditions for enzymatic clarification of banana juice using response surface methodology (RSM) [J].Journal of Food Engineering, 2006, 73 (1) :55-63.
[14]	Yan W, Cui SW, Tang J, et al.Optimization of extraction process of crude polysaccharides from boat-fruited sterculia seeds by response surface methodology[J].Food Chemistry, 2007, 105 (4) :1599-1605.
[15]	崔凤杰, 徐泓瑜, 舒畅, 等.响应曲面法优化灰树花水溶性多糖提取工艺的研究[J].食品科学, 2006, 27 (4) :142-151.
[16]	何彦峰, 杨仁明, 胡娜, 等.响应面法优化胡芦巴种子多糖提取工艺[J].天然产物开发与研究, 2012, 24 (10) :1463-1467.
[17]	王泽锋, 石玲苏一兰, 等.微波辅助提取紫薯蓣中多糖工艺研究[J].食品工业科技, 2014, 35 (4) :256-260.
[18]	王秀奇, 秦淑媛, 高天慧, 等.基础生物化学实验[M].北京:高等教育出版社, 2006:103-105.