Study on adsorption of cadmium in extract of Pinctada martensii glycosaminoglycan by attapulgite-chitosan
-
摘要: 采用凹土负载壳聚糖对马氏珠母贝全脏器的酶解液进行吸附脱镉研究,通过单因素实验,考察了振荡时间、pH、振荡速度和复合物(凹土-壳聚糖)添加量对糖胺聚糖(GAG)粗品中镉脱除和GAG含量的影响,并利用响应面法优化出镉脱除条件。结果表明:糖胺聚糖中镉脱除的最优条件是振荡时间31.0min,pH7.0,振荡速度143.0r/min,复合物添加量13.0mg/mL,在此条件下脱镉率达到76.9%,GAG含量为47.1%。Abstract: Absorption of the cadmium of hydrolyzate from Pinctada martensii whole organ by attapulgite-chitosan complex was studied. The effects of vibration time, pH, vibration speed and compound dosage (attapulgitechitosan) of attapulgite-chitosan on cadmium removal rate and GAG yield were investigated through single factor assay, and the conditions of removing cadmium were optimized by response surface method. The optimum conditions of removing cadmium in GAG were 143.0r/min of speed of vibration and pH7.0, processing for 31.0min with 13.0mg/mL dosage of attapulgite-chitosan. Under this condition, cadmium removal rate reached76.9%, GAG yield reached 47.1%.
-
Keywords:
- Pinctada martensii /
- glycosaminoglycan /
- cadmium /
- attapulgite-chitosan
-
[1] 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社, 1999:78-79. [2] 张豁中, 温玉麟.动物活性成分化学[M].天津:天津科学技术出版社, 1995:996-998. [3] Meng G T, Ching K M, Ma C Y.Thermal aggregation of globulin from an indigenous Chinese legume, Phaseolus angularis (red bean) [J].Food Chemistry, 2002, 79:93-103.
[4] 衣美艳.凹土负载壳聚糖对两种贝类糖胺聚糖中镉的吸附作用研究[D].湛江:广东海洋大学, 2012. [5] 吴红棉, 雷晓凌, 洪鹏志, 等.珠母贝糖胺聚糖的纯化及其化学性质[J].水产学报, 2000, 24 (6) :570-574. [6] 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社, 1999:11-12. [7] 食品中镉的测定方法.GB/T 5009.15—1996[S]. [8] 郭敏杰, 刘振, 李梅.壳聚糖吸附重金属离子的研究进展[J].化工环保, 2004, 24 (4) :262-265.
计量
- 文章访问数: 164
- HTML全文浏览量: 14
- PDF下载量: 107