多级溶剂萃取-气相色谱测定羊奶中致膻游离脂肪酸

杨春杰; 丁武; 马利杰

doi:10.13386/j.issn1002-0306.2014.14.005

多级溶剂萃取-气相色谱测定羊奶中致膻游离脂肪酸

西北农林科技大学食品科学与工程学院

基金项目:

国家公益性行业(农业)科研专项经费项目(3-45)；

详细信息

作者简介:
杨春杰 (1985-) , 男, 硕士研究生, 研究方向:畜产品加工和食品安全。;

中图分类号: O657.71;TS252.7
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- 收稿日期: 2013-10-08

Determination of the free fatty acids responsible for goaty flavor using multilevel solvent extraction-gas chromatography

College of Food Science and Engineering, Northwest A&F University

摘要

摘要: 目的:建立了多级溶剂萃取-气相色谱测定羊奶中致膻游离脂肪酸（己酸、辛酸、癸酸）的方法。方法:样品经过氯仿-甲醇（2∶1,v/v）溶液、碳酸钠碱性溶液多级溶剂萃取浓缩,并经三氟化硼甲醇溶液（14%,w/w）酯化后,用配备氢火焰离子化检测器（FID）的气相色谱测定。结果:己酸、辛酸、癸酸的线性范围分别为5.11¹63.52、5.05¹61.73和7.54²41.38mg/L,决定系数R2均大于0.998;三种游离脂肪酸的检出限和定量限分别在0.16⁰.25mg/L和0.54⁰.83mg/L范围内;加标回收率分别为88.5%、91.9%和103.4%,方法相对标准偏差为1.98%⁶.09%。结论:本方法经济、准确、灵敏度高,适用于液态奶产品中这几种游离脂肪酸的检测。
- 多级溶剂萃取 /
- 甲酯化 /
- 气相色谱 /
- 游离脂肪酸 /
- 羊奶
Abstract: Objective:A method for the simultaneous determination of the free fatty acids (FFA) in goat milk such as caproic acid, caprylic acid and decanoic acid, which were responsible for goaty flavor, was developed by multilevel solvent extraction-gas chromatography. Methods:Extractions were carried out with chloroform/methanol (2∶1, v/v) and sodium carbonate solution. The fatty acid methyl esters (FAMEs) which were obtained by the esterification of the FFA with boron trifluoride-methanol solution was qualified and quantified by gas chromatography equipped with flame ionization detector. Results:Over the concentration in the range of 5.11¹63.52mg/L for caproic acid, 5.05¹61.73mg/L for caprylic acid and 7.54²41.38mg/L for decanoic acid, the coefficients of the calibration curves were above 0.998. The limits of detection (LOD) were 0.16mg/L for caproic acid, 0.19mg/L for caprylic acid and 0.25mg/L for decanoic acid. The limits of quantification (LOQ) were0.54mg/L for caproic acid, 0.63mg/L for caprylic acid and 0.83mg/L for decanoic acid. The average recoveries of the three kinds of FFA ranged from 88.5% to 103.4% with relative standard deviations (RSDs) between1.98% and 6.09%. Conclusion:The method was economical, accurate, and sensitive, which may be used for determination of the FFAs in milk.
- solvent extraction /
- methylation /
- gas chromatography /
- free fatty acid /
- goat milk

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参考文献(20)

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多级溶剂萃取-气相色谱测定羊奶中致膻游离脂肪酸

作者简介: 杨春杰 (1985-) , 男, 硕士研究生, 研究方向:畜产品加工和食品安全。;

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出版历程

Determination of the free fatty acids responsible for goaty flavor using multilevel solvent extraction-gas chromatography

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出版历程

目录

作者简介:
杨春杰 (1985-) , 男, 硕士研究生, 研究方向:畜产品加工和食品安全。;