Study on the HPLC-DAD-ELSD fingerprinting of dark teas from different regions in China
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摘要: 采用二极管阵列-蒸发光散射检测器（DAD-ELSD）串联的检测技术,建立了我国不同产地黑茶的HPLC-DAD-ELSD指纹图谱。结果表明,36个黑茶样品的HPLC-DAD指纹图谱中共确定了27个共有峰,鉴别了没食子酸、表没食子儿茶素等8种组分,HPLC-ELSD指纹图谱中共确定了15个共有峰,鉴别了谷氨酸、L-茶氨酸等6种组分。相似度分析结果表明,36个黑茶样品与黑茶HPLC-DAD共有模式的相似度在0.9030.979之间,与黑茶HPLC-ELSD共有模式的相似度在0.9020.974之间;而6个对照茶样与黑茶指纹图谱共有模式的相似度均低于0.900。该研究建立的黑茶HPLC-DAD-ELSD指纹图谱能为我国黑茶的鉴别和质量评价提供参考。Abstract: The chromatographic fingerprinting of dark teas from different regions in China was established by high performance liquid chromatography using diode array and evaporative light- scattering detector. The results showed that 27 common peaks were demarcated and eight constituents, for example, gallic acid and epigallocatechin were identified in the chromatographic fingerprinting with HPLC-DAD of 36 dark tea samples; 15common peaks were demarcated and six constituents, for example, glutamicacid and L-theanine were identified in the chromatographic fingerprinting with HPLC- ELSD.The results of similarity evaluation showed that the HPLCDAD similarity of 36 dark tea samples was from 0.903 to 0.979, the HPLC- ELSD similarity of 36 dark tea samples was from 0.902 to 0.974, and the similarity of six control samples was less than 0.900. The research supplies a reference method to the identification and quality assessment of dark tea.
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