Batch fermentation kinetics of ε-Polylysine by Streptomyces Albus KD-11
-
摘要: 利用白色链霉菌（Streptomyces Albus KD-11）为出发菌株在30L全自动发酵罐中进行ε-PL分批发酵动力学研究。基于Logistic方程、Luedeking-Piret方程、类似Luedeking-Piret方程建立了Streptomyces Albus KD-11菌体生长、ε-PL产物合成和底物葡萄糖消耗的动力学模型。利用Origin 8.1软件对模型参数进行非线性拟合,建立的模型拟合程度R2均大于0.980,拟合良好。当葡萄糖初始浓度在4555g/L范围内,实验数据与模型预测值平均相对误差小于8%,适用性良好。表明该动力学模型对指导ε-PL的发酵工艺优化具有指导意义。Abstract: The kinetic models of the batch fermentation of ε-PL by Streptomyces Albus KD-11 in a 30L-bioreactor were studied. The kinetics model was proposed by using the Logistic equation for cell growth, the Luedeking Piret equation for ε-PL production and the Luedeking-Piret-like equation for consumption of glucose as substrate. Parameters were gained with nonlinear curve fit by Origin 8.1 version. The goodness-of-fit scores R2 of models are all greater than 0.980 showed good agreement of predicted values with the experimental values.Calculated results of models were compared satisfactorily with experimental data under various initial glucose concentrations (4555g/L) , the average relative error was no more than 8%. The kinetic model had guiding process in ε-PL fermentation of Streptomyces Albus KD-11.
-
Keywords:
- Streptomyces Albus /
- batch fermentation /
- kinetics model /
- ε-Polylysine
-
[1] Shima S, Sakai H.Poly-L-lysine produced by Streptomyces.Part III.Chemical studies[J].Agric Biol-Chem, 1981, 45:2503-2508.
[2] Hiraki J.ε-Polylysine:it’s development and utilization[J].Fine Chem, 2000, 29:18-25.
[3] Hirohara H, Takehara M, Saimura M, et al.Biosynthesis of poly (ε-L-lysine) s in two newly isolated strains of Streptomyces sp.[J].Appl Microbiol Biotechnol, 2006, 739 (11) :321-331.
[4] Hiraki J, Ichikawa T, Ninomiya S, et al.Barnett.Use of ADME studies to confirm the safety ofε-polylysine as a preservative in food[J].Regul Toxicol Pharmacol, 2003, 37:328-340.
[5] Hamano Y, Nicchu I, Hoshino Y, et al.Development of gene delivery systems for theε-poly-L-lysine producer, Streptomyces albulus[J].Biosci Bioeng, 2005, 99:636-641.
[6] 李述日, 吴清平, 吴军林.利用细菌开发天然功能性食品添加剂研究进展[J].食品工业科技, 2011, 32 (2) :425-429. [7] 戚以政, 夏杰.生物反应工程[M].北京:化学工业出版社, 2007. [8] 张建国, 陈晓明, 熊双丽.响应面分析优化ε-聚赖氨酸发酵培养基[J].食品与机械, 2010, 26 (4) :19-22. [9] Itzhaki R F.Colorimetric method for estimating Poly-lysine and Poly-arginine[J].Anal Biochem, 1972, 50:569-574.
[10] Miller G L.Use of dinitrosalicylic acid reagent for determination of reducing sugar[J].Anal Chem, 1959, 31 (3) :426-428.
[11] 武新, 张永胜.Origin在曲线拟合中的应用[J].计算机工程与应用, 2005 (17) :206-208. [12] Kahar P, Iwata T, Hiraki J, et al.Enhancement ofε-polylysine production by Streptomyces albulus strain 410 using pH control[J].Journal of Bioscience and Bioengineering, 2001, 91 (2) :190-194.
[13] 张建国, 陈晓明, 贺新生.灵芝胞外多糖分批发酵动力学模型[J].生物工程学报, 2007, 23 (6) :1065-1070. [14] 贾士儒.生物反应工程原理[M].天津:南开大学出版社, 2003.
计量
- 文章访问数: 112
- HTML全文浏览量: 16
- PDF下载量: 120
下载:
