响应面法优化超声波辅助提取葡萄悬浮细胞花色苷

焦彩凤; 俞超; 吴月燕

doi:10.13386/j.issn1002-0306.2014.11.040

响应面法优化超声波辅助提取葡萄悬浮细胞花色苷

浙江万里学院生物与环境学院

基金项目:

宁波市科技创新团队项目(2011B82019)；宁波市科技项目(2010C91049)；浙江省科技厅项目(2011C32008)；

详细信息

作者简介:
焦彩凤 (1990-) , 女, 硕士研究生, 研究方向:食品化学工程与安全控制。;

中图分类号: TQ914.1
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- 文章访问数: 144
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出版历程
- 收稿日期: 2013-10-29

Optimization of ultrasound-assisted extraction of anthocyanin in Vitis vinifera suspension cultures by response surface methodology

College of Biological & Environmental Sciences, Zhejiang Wanli University

摘要

摘要: 采用响应面法对葡萄悬浮细胞花色苷的提取工艺参数进行优化。在单因素实验基础上,选取酸化乙醇浓度（含体积分数为0.1%的盐酸）、超声功率、提取时间和提取温度为自变量,以花色苷含量为响应值,利用Box-Behnken中心组合设计原理和响应面分析法,研究各自变量及其交互作用对花色苷含量的影响,并优化提取工艺。结果表明,提取的最佳工艺条件为:料液比1∶9、酸化乙醇浓度85%、超声功率300W、提取时间38min、提取温度42℃。在此条件下获得的花色苷提取产量预测值为3.96mg/L,与实测值3.93mg/L相符。超声波技术适用于葡萄悬浮细胞花色苷的提取,所拟合的统计模型可信度高。本研究结果对工厂化生产葡萄花色苷的研究具有较好的借鉴意义。
- 葡萄悬浮细胞 /
- 花色苷 /
- 超声波 /
- 提取 /
- 响应面分析法
Abstract: Response surface methodology ( RSM) was used to optimize extraction of anthocyanin from Vitis vinifera suspension cultures.Based on single factor tests, acidic ethanol concentration ( containing 0.1% hydrochloric acid, V/V) , ultrasonic power, extraction time and extraction temperature were chosen as arguments and the content of anthocyanin as response value.The interaction of the respective variables and their influence on the content of anthocyanin and the optimum process were studied by using Box- Behnken central composite design and response surface analysis theory.The results showed that the optimum conditions were: liquid- solid ratio of 1∶9, acidic ethanol concentration 85%, ultrasonic power 300W, extraction time 38min and extraction temperature 42℃ for twice.Under these conditions, the content of anthocyanins was up to 3.96mg /L, which was consistent with the measured value ( 3.93mg /L) .The results indicated that ultrasound technology was suitable for the extraction of anthocyanins in Vitis Vinifera suspension cultures, and the statistical model fitting was with high reliability.This study has some practical significance for the research of the industrialized production of anthocyanins.
- Vitis Vinifera suspension cultures /
- anthocyanins /
- ultrasound /
- extraction /
- response surface method

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参考文献(16)

[1]	孙欣, 韩键, 房经贵, 等.葡萄浆果着色分子机理的重要研究进展[J].植物生理学报, 2012, 48 (4) :333-342.
[2]	吴斌, 张玉丽, 程琳琳, 等.鲜食葡萄花色苷的研究进展[J].现代食品科技, 2012, 28 (4) :441-444.
[3]	Cooper-Driver G A.Contributions of Jeffrey Harborne and co-workers to the study of anthocyanins[J].Phytochemistry, 2001, 56 (3) :229-236.
[4]	Kanner J, Frankel E, Granit R, et al.Natural antioxidants in grapes and wines[J].Journal of Agricultural and Food Chemistry, 1994, 42 (1) :64-69.
[5]	Pietta P G.Flavonoids as antioxidants[J].Journal of Natural Products, 2000, 63 (7) :1035-1042.
[6]	Zhang W, Curtin C, Kikuchi M, et al.Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures[J].Plant Science, 2002, 162 (3) :459-468.
[7]	Li Z, Pan Q H, Cui X G, et al.Optimization on anthocyanins extraction from wine grape skins using orthogonal test design[J].Food Science and Biotechnology, 2010, 19 (4) :1047-1053.
[8]	Kharenko O A, Zaharia L I, Giblin M, et al.Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri[J].Plant Cell, Tissue and Organ Culture (PCTOC) , 2011, 105 (3) :415-422.
[9]	Lapornik B, Proek M, Golc Wondra A.Comparison of extracts prepared from plant by-products using different solvents and extraction time[J].Journal of Food Engineering, 2005, 71 (2) :214-222.
[10]	Wang L, Weller C L.Recent advances in extraction of nutraceuticals from plants[J].Trends in Food Science&Technology, 2006, 17 (6) :300-312.
[11]	Wang J, Sun B, Cao Y, et al.Optimisation of ultrasoundassisted extraction of phenolic compounds from wheat bran[J].Food Chemistry, 2008, 106 (2) :804-810.
[12]	Rostagno M A, Palma M, Barroso C G.Ultrasound-assisted extraction of soy isoflavones[J].Journal of Chromatography A, 2003, 1012 (2) :119-128.
[13]	苗颖, 吴淑娟.超声波法提取葡萄皮色素[J].食品研究与开发, 2009, 30 (3) :190-192.
[14]	王振忠, 武文洁.野菊花总黄酮提取工艺的响应面设计优化[J].时珍国医国药, 2007, 18 (3) :648-650.
[15]	戚向阳, 姚叶斌.杨梅花色苷提取工艺条件的响应面分析及其抗氧化活性[J].果树学报, 2009, 26 (2) :226-230.
[16]	Mihai R, Mitoi M, Brezeanu A, et al.Two-stage system, a possible strategy for the enhancement of anthocyanin biosynthesis in a long-term grape callus cultures[J].Romanian Biotechnological Letters, 2010, 15 (1) :5025-5033.