响应面法优化微波辅助提取红皮云杉多酚工艺

周芳; 赵鑫; 宫婕; 刘海鹏; 王振宇

doi:10.13386/j.issn1002-0306.2014.10.052

响应面法优化微波辅助提取红皮云杉多酚工艺

周芳^1,2,
赵鑫¹,
宫婕¹,
刘海鹏¹,
王振宇³

1. 东北林业大学林学院
2. 黑龙江省质量监督检测研究院
3. 哈尔滨工业大学食品学院

基金项目:

国家自然科学基金资助项目(31170510)；

详细信息

作者简介:
周芳 (1981-) , 女, 博士, 主要从事植物资源开发及植物活性成分方面的研究。;

中图分类号: TQ914.1
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出版历程
- 收稿日期: 2013-11-10

Optimization of microwave-assisted extraction conditions of polyphenol from Picea koraiensis Nakai by response surface methodology

School of Forestry, Northeast Forestry University
The Academy of Quality Inspection and Research in Hei Long Jiang Province
School of Food Science and Engineering, Harbin Institute of Technology

摘要

摘要: 采用响应面法优化微波辅助提取红皮云杉多酚工艺。在单因素实验的基础上,选择料液比、微波功率、提取时间为自变量,以多酚得率为响应值,采用Box-Benhnken法设计三因素三水平的响应分析实验。结果表明,回归模型拟合度高,最佳工艺条件为:料液比1∶31（g/mL）、微波功率295W、提取时间46s,乙醇浓度40%。在此条件下,红皮云杉多酚得率的预测值为17.51%,实际值为17.38%,两者相对误差<1%,表明该工艺条件合理可行。
- 红皮云杉 /
- 响应面 /
- 微波 /
- 多酚
Abstract: The microwave-assisted extraction conditions of polyphenol from Picea koraiensis was optimized by response surface methodology. Based on single factor test, a 3-factor, 3-level Box-Behnken experimental was adopted, including dependent variables (ratio of material to liquid, microwave power and extraction time) and independent variable (yield of polyphenols) . Results showed that the regression model fit well with experimental data. The optimum conditions were ratio of material to liquid 1∶31 (g/mL) , microwave power 295W, extraction time 46s, ethanol concentration 40%. Under these conditions, the predicted value of yield of polyphenol was 17.51%, compared to the measured value 17.38%, the relative error<1%. Result showed that the conditions were reasonable and feasible.
- Picea koraiensis Nakai /
- response surface methodology /
- microwave /
- polyphenol

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参考文献(13)

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