Study on ultrasonic extraction of seed watermelon polysaccharides and evaluation of its molecular weight and antioxidant activity
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摘要: 采用超声波辅助萃取技术从籽瓜中提取籽瓜多糖（SWP）,在单因素实验基础上进行响应面实验,对影响籽瓜多糖得率的提取时间、提取温度和料液比三个因素进行优化,结果显示:最优工艺参数为超声时间85min、温度49℃、料液比1∶1.7（g/mL）,多糖得率为4.128%。采用体积排阻色谱-光散射联用测得籽瓜多糖的分子量为Mn=1.089×106g/mol。通过测定籽瓜多糖对自由基的清除率,结果显示:在浓度为0.010.32mg/mL对DPPH自由基的清除作用明显高于VC,可达到68%;在浓度为5mg/mL时,对羟基（·OH）自由基的清除率达到75.35%;而对超氧阴离子（O2-·）,在浓度为5mg/mL时,清除率达到50%,表明籽瓜多糖具有较强的自由基清除作用,可作为潜在天然抗氧化剂应用于食品和医药工业中。Abstract: The seed watermelon polysaccharide was extracted from seed watermelon using ultrasonic assisted extraction technique. The three factors:extraction time, extraction temperature and solid-liquid ratio were optimized based on response surface methodology ( RSM) . The results showed that the optimum seed watermelon polysaccharides extraction conditions was:ultrasonic treatment time 85min, extraction temperature 49℃, and solid/liquid ratio 1 ∶1.7g/mL, seed watermelon polysaccharides yield reached up to 4.128%. The molecular weight of seed watermelon polysaccharides was measured using SEC-HPLC and LLS:Mn=1.089×106g/moL. According to the scavenging capacity of seed watermelon polysaccharides against radicals, it showed that the scavenging capacity of seed watermelon polysaccharides agaist DPPH radicals was 68% at the concentration of 0.010.32mg/mL, higher than VCsignificantly. The scavenging capacity of seed watermelon polysaccharides agaist hydroxyl radicals was 75.35% at the concentration of 5mg/mL and the scavenging capacity of superoxide radicals was 50% at the same concentration. The result showed that seed watermelon polysaccharides had strong free radical-scavenging capacity. And could be use as a potential natural antioxidant in the food and pharmaceutical industry.
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