籽瓜多糖的超声波辅助提取工艺、分子量和抗氧化活性研究

宋珅; 刘琴; 殷振雄; 赵保堂; 张继

doi:10.13386/j.issn1002-0306.2014.10.051

籽瓜多糖的超声波辅助提取工艺、分子量和抗氧化活性研究

宋珅¹,
刘琴¹,
殷振雄¹,
赵保堂¹,
张继^1,2

1. 西北师范大学生命科学学院
2. 甘肃特色植物有效成分制品工程技术研究中心

基金项目:

国家科技支撑计划(2012BAD36B04)；

详细信息

作者简介:
宋珅 (1988-) , 男, 硕士研究生, 研究方向:天然产物研究与开发。;

中图分类号: TQ914.1
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- 文章访问数: 133
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出版历程
- 收稿日期: 2013-08-29

Study on ultrasonic extraction of seed watermelon polysaccharides and evaluation of its molecular weight and antioxidant activity

College of Life Science, Northwest Normal University
Research Center For Gansu Distinctive Plants, Northwest Normal University

摘要

摘要: 采用超声波辅助萃取技术从籽瓜中提取籽瓜多糖（SWP）,在单因素实验基础上进行响应面实验,对影响籽瓜多糖得率的提取时间、提取温度和料液比三个因素进行优化,结果显示:最优工艺参数为超声时间85min、温度49℃、料液比1∶1.7（g/mL）,多糖得率为4.128%。采用体积排阻色谱-光散射联用测得籽瓜多糖的分子量为Mn=1.089×106g/mol。通过测定籽瓜多糖对自由基的清除率,结果显示:在浓度为0.01⁰.32mg/mL对DPPH自由基的清除作用明显高于VC,可达到68%;在浓度为5mg/mL时,对羟基（·OH）自由基的清除率达到75.35%;而对超氧阴离子（O2-·）,在浓度为5mg/mL时,清除率达到50%,表明籽瓜多糖具有较强的自由基清除作用,可作为潜在天然抗氧化剂应用于食品和医药工业中。
- 籽瓜多糖 /
- 超声波辅助提取 /
- 分子量 /
- 抗氧化活性
Abstract: The seed watermelon polysaccharide was extracted from seed watermelon using ultrasonic assisted extraction technique. The three factors:extraction time, extraction temperature and solid-liquid ratio were optimized based on response surface methodology ( RSM) . The results showed that the optimum seed watermelon polysaccharides extraction conditions was:ultrasonic treatment time 85min, extraction temperature 49℃, and solid/liquid ratio 1 ∶1.7g/mL, seed watermelon polysaccharides yield reached up to 4.128%. The molecular weight of seed watermelon polysaccharides was measured using SEC-HPLC and LLS:Mn=1.089×106g/moL. According to the scavenging capacity of seed watermelon polysaccharides against radicals, it showed that the scavenging capacity of seed watermelon polysaccharides agaist DPPH radicals was 68% at the concentration of 0.01⁰.32mg/mL, higher than VCsignificantly. The scavenging capacity of seed watermelon polysaccharides agaist hydroxyl radicals was 75.35% at the concentration of 5mg/mL and the scavenging capacity of superoxide radicals was 50% at the same concentration. The result showed that seed watermelon polysaccharides had strong free radical-scavenging capacity. And could be use as a potential natural antioxidant in the food and pharmaceutical industry.
- seed watermelon polysaccharide /
- ultrasonic assisted extraction /
- molecular weight /
- antioxidant activity

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