花椒籽仁油多不饱和脂肪酸乙酯的制备及氧化稳定性研究

边凤霞; 姚世勇; 刘荣; 殷钟意; 郑旭煦

doi:10.13386/j.issn1002-0306.2014.10.050

花椒籽仁油多不饱和脂肪酸乙酯的制备及氧化稳定性研究

1. 重庆工商大学环境与生物工程学院
2. 重庆工商大学催化与功能有机分子重庆市重点实验室

基金项目:

重庆市科技攻关项目(CSTC2011AB5007)；重庆高校创新团队建设计划(KJTD201020)；

详细信息

作者简介:
边凤霞 (1987-) , 女, 硕士研究生, 助教, 主要从事环境生物工程方面的研究。;

中图分类号: TQ461;R285
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出版历程
- 收稿日期: 2013-07-30

Study on preparation and oxidation stability of polyunsaturated fatty acid ethyl ester from Zanthoxylum seeds kernel oil

Environmental and Biological Engineering Institute, Chongqing Technology and Business University
Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University

摘要

摘要: 为提高花椒籽仁油的降脂活性和氧化稳定性,将其制备成富含α-亚麻酸乙酯的多不饱和脂肪酸乙酯（PUFAEE）。以自制的花椒籽仁油为原料,以α-亚麻酸乙酯产率为检测指标,优化碱催化法制备混合脂肪酸乙酯的工艺,利用硝酸银络合法分离纯化出PUFAEE,测定其氧化诱导时间（OIP）。结果表明,在乙醇钠用量为0.5%（wt%）、醇油体积比为6∶1、反应温度为60℃、反应时间为60min的条件下制备混合脂肪酸乙酯,α-亚麻酸乙酯产率达94.69%;分离纯化的PUFAEE富含α-亚麻酸乙酯（76.9%）,氧化稳定性好,其OIP为（5.39±0.07）h,远远高于花椒籽仁油的OIP（0.62±0.05）h;芝麻酚可作为花椒籽仁油及其PUFAEE的抗氧化剂,其强化作用呈现一定的量效关系。
- 花椒籽仁油 /
- 多不饱和脂肪酸乙酯 /
- 制备 /
- 氧化稳定性 /
- 氧化诱导时间
Abstract: In order to improve the lipid-lowering activity and oxidation stability of Zanthoxylum seed kernel oil, the polyunsaturated fatty acid ethyl ester (PUFAEE) being rich in α-linolenic acid ethyl ester was prepared. Using self-made Zanthoxylum seed kernel oil as raw material, with the yield of α-linolenic acid ethyl ester as detection index, the process preparing mixed fatty acid ethyl ester by using alkali catalyzed method was optimized, PUFAEE was separation and purification by using silver nitrate complexation method, and its oxidative induction period (OIP) was determined. The results showed that the mixed fatty acid ethyl ester was prepared and the yield of α-linolenic acid ethyl ester was 94.69% when the sodium ethylate was 0.5% (wt%) , the volume ratio of alcohol and oil was 6∶1, the reaction temperature was 60℃ and the reaction time was 60min. The prepared PUFAEE were rich in α-linolenic acid ethyl ester (76.9%) , its oxidation stability was good, its OIP was (5.39±0.07) h, far higher than the OIP of Zanthoxylum seed kernel oil (0.62±0.05) h. Sesamol could be used as antioxidant of Zanthoxylum seed kernel oil and their PUFAEE, their strengthening effect showed a doseresponse relationship to some degree.
- Zanthoxylum seed kernel oil /
- polyunsaturated fatty acid ethyl ester /
- preparation /
- oxidation stability /
- oxidative induction period

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