花椒籽仁油多不饱和脂肪酸乙酯的制备及氧化稳定性研究

边凤霞; 姚世勇; 刘荣; 殷钟意; 郑旭煦

doi:10.13386/j.issn1002-0306.2014.10.050

花椒籽仁油多不饱和脂肪酸乙酯的制备及氧化稳定性研究

1. 重庆工商大学环境与生物工程学院
2. 重庆工商大学催化与功能有机分子重庆市重点实验室

基金项目:

重庆市科技攻关项目(CSTC2011AB5007)；重庆高校创新团队建设计划(KJTD201020)；

详细信息

作者简介:
边凤霞 (1987-) , 女, 硕士研究生, 助教, 主要从事环境生物工程方面的研究。;

中图分类号: TQ461;R285
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出版历程
- 收稿日期: 2013-07-30

Study on preparation and oxidation stability of polyunsaturated fatty acid ethyl ester from Zanthoxylum seeds kernel oil

Environmental and Biological Engineering Institute, Chongqing Technology and Business University
Chongqing Key Lab of Catalysis & Functional Organic Molecules, Chongqing Technology and Business University

摘要

摘要: 为提高花椒籽仁油的降脂活性和氧化稳定性,将其制备成富含α-亚麻酸乙酯的多不饱和脂肪酸乙酯（PUFAEE）。以自制的花椒籽仁油为原料,以α-亚麻酸乙酯产率为检测指标,优化碱催化法制备混合脂肪酸乙酯的工艺,利用硝酸银络合法分离纯化出PUFAEE,测定其氧化诱导时间（OIP）。结果表明,在乙醇钠用量为0.5%（wt%）、醇油体积比为6∶1、反应温度为60℃、反应时间为60min的条件下制备混合脂肪酸乙酯,α-亚麻酸乙酯产率达94.69%;分离纯化的PUFAEE富含α-亚麻酸乙酯（76.9%）,氧化稳定性好,其OIP为（5.39±0.07）h,远远高于花椒籽仁油的OIP（0.62±0.05）h;芝麻酚可作为花椒籽仁油及其PUFAEE的抗氧化剂,其强化作用呈现一定的量效关系。
- 花椒籽仁油 /
- 多不饱和脂肪酸乙酯 /
- 制备 /
- 氧化稳定性 /
- 氧化诱导时间
Abstract: In order to improve the lipid-lowering activity and oxidation stability of Zanthoxylum seed kernel oil, the polyunsaturated fatty acid ethyl ester (PUFAEE) being rich in α-linolenic acid ethyl ester was prepared. Using self-made Zanthoxylum seed kernel oil as raw material, with the yield of α-linolenic acid ethyl ester as detection index, the process preparing mixed fatty acid ethyl ester by using alkali catalyzed method was optimized, PUFAEE was separation and purification by using silver nitrate complexation method, and its oxidative induction period (OIP) was determined. The results showed that the mixed fatty acid ethyl ester was prepared and the yield of α-linolenic acid ethyl ester was 94.69% when the sodium ethylate was 0.5% (wt%) , the volume ratio of alcohol and oil was 6∶1, the reaction temperature was 60℃ and the reaction time was 60min. The prepared PUFAEE were rich in α-linolenic acid ethyl ester (76.9%) , its oxidation stability was good, its OIP was (5.39±0.07) h, far higher than the OIP of Zanthoxylum seed kernel oil (0.62±0.05) h. Sesamol could be used as antioxidant of Zanthoxylum seed kernel oil and their PUFAEE, their strengthening effect showed a doseresponse relationship to some degree.
- Zanthoxylum seed kernel oil /
- polyunsaturated fatty acid ethyl ester /
- preparation /
- oxidation stability /
- oxidative induction period

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参考文献(21)

[1]	SCISLOWSKI V, BANCHART D, GRUFAT D, et al.Efect of dietary n-6 and n-3 polyunsaturated fatty acids on poroxidizability of lipoproteins in steers[J].Lipids, 2005, 40 (12) :1245-1246.
[2]	Nirmala K, Prasanna K, Kalpagam P.Alterations in antioxidant status of rats following intake of ginger through diet[J].Food Chemistry, 2007, 106:991-996.
[3]	禹晓, 邓乾春, 黄凤洪, 等.不同α-亚麻酸含量油脂对高脂模型大鼠脂质水平及氧化损伤的影响[J].营养学报, 2011, 33 (2) :129-137.
[4]	郑旭煦, 殷钟意, 杜若愚, 等.同种溶剂两步浸提法分离提取花椒籽皮油和仁油[J].食品科学, 2010, 31 (22) :180-184.
[5]	刘永英, 王多宁, 刘渊声, 等.花椒籽仁油对实验性高脂血症大鼠的防治作用[J].第四军医大学学报, 2007, 28 (5) :411-413.
[6]	ARMENTA R E, VINATORU M, BUR JA A M, et al.Transesterification of fish oil to produce fatty acid ethyl estersusingultrasonic energy[J].Jam oil Chem Sic, 2007, 84:1045-1052.
[7]	侯继贤, 鲁吉珂, 聂开立, 等.固定化Candida sp.99-125脂肪酶催化大豆油合成脂肪酸乙酯[J].过程工程学报, 2008, 8 (2) :355-358.
[8]	刘润哲, 彭桦, 梅连平, 等.脂肪酸乙酯的制备与纯化[J].粮食与食品工业, 2010, 5 (17) :22-25.
[9]	ZHOU WY, KONAR S K, BOOCOCK D G B.Ethyl esters from the single phase base-catalyzed ethanolysis of vegetable oils[J].Jam oil Chem Sic, 2003, 80:367-371.
[10]	陈伟珠, 洪专, 赵敏, 等.鱼油中二十碳五烯酸乙酯和二十二碳六烯酸乙酯的超临界流体色谱分离技术[J].食品科学, 2012, 33 (24) :82-85.
[11]	唐君, 张春枝, 吴文忠.一种鱼油中EPA和DHA富集的新方法[J].大连工业大学学报, 2008, 27 (2) :102-104.
[12]	周晓丹, 王妍, 刘晶, 等.橄榄油、葵花籽油和米糠油的氧化稳定性[J].食品科学, 2011, 32 (13) :119-121.
[13]	CHRISTIAN G, SABINE K, PARKASH K S.Testing and comparing oxidative stability of vegetable oil and fats at frying temperature[J].European Journal of Lipid Science and Technology, 2000, 102 (8/9) :543-551.
[14]	NAZ S, SHEIKH H, SIDDIQI R, et al.Oxidative stability of olive, corn and soybean oil under different conditions[J].Food Chemistry, 2004, 88 (2) :253-254.
[15]	TONU P, REGINA P, PIRET R, et al.Inhibition of lipid oxidation and dynamics of polyphenol content in mechanically deboned meat supplement with sea buckthorn (Hippophae rhamnoides) berry residues[J].Food Chemistry, 2008, 107:714-716.
[16]	李瑞贞, 刘玉环, 周厚德, 等.光皮树油氧化稳定性的研究[J].食品科学, 2009, 30 (21) :87-89.
[17]	边凤霞, 郑旭煦, 殷钟意, 等.压榨花椒籽仁油的制备及氧化稳定性[J].食品科学, 2013, 34 (16) :46-50.
[18]	夏辉, 鲁海龙.元宝枫油乙酯制备工艺[J].中国油脂, 2010, 35 (6) :26-28.
[19]	蒋艳忠, 胡小明, 杨克迪.硝酸银络合法浓缩蚕蛹油中α-亚麻酸的研究[J].食品工业科技, 2008, 29 (4) :205-209.
[20]	邓乾春, 黄庆德, 黄凤洪, 等.亚麻籽油调和油的热稳定性研究[J].食品科学, 2012, 33 (5) :88-92.
[21]	黄克, 崔春, 赵谋明, 等.天然抗氧化剂的增效作用及其对花生油抗氧化效果研究[J].现代食品科技, 2012, 28 (9) :1139-1141.

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