高效液相色谱法同时测定羊肉中的11种镇静剂类药物

魏晋梅; 罗玉柱; 白云旭

doi:10.13386/j.issn1002-0306.2014.10.012

高效液相色谱法同时测定羊肉中的11种镇静剂类药物

1. 甘肃农业大学研究测试中心
2. 甘肃省草食动物生物技术重点实验室
3. 甘肃农业大学理学院

基金项目:

甘肃省自然科学基金计划课题(1208RJZA156)；

详细信息

作者简介:
魏晋梅 (1973-) , 女, 硕士研究生, 副研究员, 研究方向:食品科学。;

中图分类号: O657.72;TS251.7
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出版历程
- 收稿日期: 2013-09-26

Simultaneous high performance liquid chromatographic determination of 11 common sedatives in mutton

Instrumental Research and Analysis Center, Gansu Agricultural University
Gansu Key Laboratory of Herbivorous Animal Biotechnology
College of Natural Sciences, Gansu Agricultural University

摘要

摘要: 建立了高效液相色谱法同时测定羊肉中的11种镇静剂类药物的方法。样品用1%氨水-乙腈溶液超声提取;色谱柱为ZORBAX Eclipse Plus C18（250mm×4.6mm,5μm）,以甲醇-0.1%甲酸为流动相进行梯度洗脱,检测波长为245nm和254nm,进样量10μL;甲苯噻嗪、唑吡坦、咔唑心安、氟哌啶醇、氯氮卓、丙酰丙嗪、氯丙嗪、奋乃静、氟奋乃静在0.02²00μg/mL范围有良好的线性关系,奥沙西泮和氟哌利多分别在0.005²00μg/mL和0.05²00μg/mL范围有良好的线性关系。11种药物的平均回收率在64.0%¹21.3%,相对标准偏差小于20%（n=6）。
- 高效液相色谱法 /
- 羊肉 /
- 镇静剂
Abstract: A method had been developed for the simultaneous determination of 11 common sedatives in mutton by high performance liquid chromatography. After being extracted by 1% ammonium hydroxide-acetonitrile from samples, 11 compounds were separated on a ZORBAX Eclipse Plus C18 (250mm×4.6mm, 5μm) column using methanol-0.1% formic acid as mobile phase with linear gradient elution, and detected by photodiode array detector (PDAD) at wavelengs of 254nm and 245nm. There were good linear correlations between the concentrations and peak areas of the 11 sedatives. The linear ranges of xylazine, zolpidem tartrate, carazolol, haloperidol, chlordiazepoxide, propionylpromazine hydrochlorid, chlorpromazine, perphenazine and fluphenazine hydrochloride were 0.02²00μg/mL. The linear ranges of oxazepam and droperidol were 0.005²00μg/mL and 0.05 ²00μg/mL, respectively. The average recoveries were 64.0% ¹21.3% with relative standard deviations below 20% (n=6) .
- HPLC /
- mutton /
- sedative

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参考文献(15)

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