Abstract: A method had been developed for the simultaneous determination of 11 common sedatives in mutton by high performance liquid chromatography. After being extracted by 1% ammonium hydroxide-acetonitrile from samples, 11 compounds were separated on a ZORBAX Eclipse Plus C18 (250mm×4.6mm, 5μm) column using methanol-0.1% formic acid as mobile phase with linear gradient elution, and detected by photodiode array detector (PDAD) at wavelengs of 254nm and 245nm. There were good linear correlations between the concentrations and peak areas of the 11 sedatives. The linear ranges of xylazine, zolpidem tartrate, carazolol, haloperidol, chlordiazepoxide, propionylpromazine hydrochlorid, chlorpromazine, perphenazine and fluphenazine hydrochloride were 0.02²00μg/mL. The linear ranges of oxazepam and droperidol were 0.005²00μg/mL and 0.05 ²00μg/mL, respectively. The average recoveries were 64.0% ¹21.3% with relative standard deviations below 20% (n=6) .