紫外分光光度法和高效液相色谱法测定Maillard体系中抗坏血酸含量的比较

唐乐攀; 周永妍; 余爱农

doi:10.13386/j.issn1002-0306.2014.10.008

紫外分光光度法和高效液相色谱法测定Maillard体系中抗坏血酸含量的比较

1. 生物资源保护与利用湖北省重点实验室
2. 湖北民族学院化学与环境工程学院

基金项目:

国家自然科学基金资助项目(31360408)；

详细信息

作者简介:
唐乐攀 (1989-) , 男, 硕士研究生, 研究方向:食品化学。;

中图分类号: O657;TS207.3
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出版历程
- 收稿日期: 2013-08-26

Comparison of UV spectrophotometry and HPLC in determination of ascorbic acid in Maillard reaction

Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province
School of Chemistry & Environmental Engineering, Hubei Minzu University

摘要

摘要: 在抗坏血酸/半胱氨酸Maillard体系中,探讨了简单、快速、准确测定抗坏血酸含量的方法。用偏磷酸溶液作为提取剂,比较了紫外分光光度法和高效液相色谱法(HPLC)测定Maillard体系中抗坏血酸的含量。结果表明:紫外分光光度法和HPLC标准曲线线性关系良好,线性范围分别为0~14μg/mL和0~100μg/mL,两种方法相关系数R均达到0.999,平均回收率分别为111%和99.7%。使用两种方法测定三组平行样时,结果相近,误差较小。说明Maillard体系中紫外分光光度法测定抗坏血酸含量时干扰较小,且相比之下其更为简单快捷,适合Maillard体系中的抗坏血酸含量的测定。
- 紫外分光光度法 /
- 高效液相色谱法 /
- Maillard体系 /
- 抗坏血酸
Abstract: The paper explored a simple, rapid and accurate method for the determination of ascorbic acid (VC) in Maillard reaction from VC-Cys model system. UV spectrophotometry and HPLC methods were compared using the metaphosphoric acid as the suitable extraction solution. The results showed that both UV spectrophotometry and HPLC methods had good linear correlations, over the measurement range of 0¹4μg/mL and 0¹00μg/mL respectively, with R reached 0.999 in both cases. The average recoveries were 111% and 99.7% respectively. The results of two methods determining 3 parallel samples were close. The error was allowed. By contrast, the UV spectrophotometry was much more simple and rapid, so it was more suitable for the determination of VCin Maillard system.
- UV spectrophotometry /
- HPLC /
- Maillard system /
- ascorbic acid

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参考文献(13)

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