闪式提取香菇柄中麦角甾醇工艺研究

史德芳; 高虹; 程薇; 范秀芝; 乔宇; 周康

doi:10.13386/j.issn1002-0306.2014.09.037

闪式提取香菇柄中麦角甾醇工艺研究

湖北省农业科学院农产品加工与核农技术研究所/国家食用菌加工技术研发分中心/湖北省农业科技创新中心农产品加工分中心

基金项目:

公益性行业(农业)专项(201303080)；湖北省农业科技创新中心资助项目(2013-620-007-001)；

详细信息

作者简介:
史德芳 (1979-) , 男, 硕士, 助理研究员, 研究方向:药 (食) 用菌功能食品开发研究。;

中图分类号: TS209
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出版历程
- 收稿日期: 2013-09-21

Flash extraction for ergosterol from Lentinula edodes stipe

Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences / National R&D Center For Edible Fungi Processing / Agricultural Products Processing Subcenter of Hubei Agricultural Science&Technology Innovation Center

摘要

摘要: 为实现麦角甾醇物质的快速高效利用,考察了闪式提取香菇柄麦角甾醇的工艺,研究了液料比、处理时间和处理电压等因素对麦角甾醇提取效果的影响,在单因素实验的基础上,对闪式提取麦角甾醇最佳条件进行了优化,并与传统热回流法、超声波辅助法进行了比较。优化结果:液料比为20mL/g、提取时间为4min,提取电压为100V,在此条件下,香菇柄麦角甾醇提取率达到2.762mg/g,与传统热回流提取法、超声波提取法相比,闪式提取法显著地降低了单位提取能耗,缩短了提取时间。
- 香菇柄 /
- 闪式提取 /
- 麦角甾醇
Abstract: Flash extraction was used to extract the ergosterol from Lentinula edodes stipe.The extraction process was optimized and the results were compared with those of conventional reflux extraction and ultrasonic extraction. The effects of three factors such as liquid- solid ratio, extraction time, extraction voltage on ergosterol extraction yield were studied by orthogonal method.The optimum extraction conditions were as follows: liquid- solid ratio 20mL /g, extracting 4min, voltage 100V, under the conditions the extraction yield of ergosterol was 2.762mg /g, compared with other two methods, flash extraction had the remarkable superiority in reducing energy consumption and saving time.
- Lentinula edodes stipe /
- flash extraction /
- ergosterol

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参考文献(16)

[1]	史徳芳, 周明, 郭鹏, 等.气流和机械碾轧超微粉碎香菇柄的效果比较[J].农业工程学报, 2012, 28 (8) :280-286.
[2]	Zhang ZP, Song HG, Peng Z, et al.Characterization of stipe and cap powders of mushroom (Lentinus edodes) prepared by different grinding methods[J].Journal of Food Engineering, 2012 (9) :406-413.
[3]	袁旭.李建荣.香菇柄风味食品的研制[J].食品工业科技, 2001, 22 (1) :43-45.
[4]	李文香, 李经道, 张华云.香菇异味成分的分离及香菇脯加工工艺[J].食品工业科技, 1998, 4:41-43.
[5]	王谦, 徐彩芳, 王朝江.微波法提取香菇柄多糖初步研究[J].食用菌学报, 2009, 16 (4) :58-60.
[6]	李莉.香菇丁气流膨化工艺及加工技术研究[D].福州:福建农林大学, 2010.
[7]	吴关威.香菇柄中滋味成分释放研究及香菇精的研制[D].武汉:华中农业大学, 2010.
[8]	黄茂坤.香菇柄膳食纤维的改性及仿真食品研制[D].福州:福建农林大学, 2008.
[9]	王小红.食用菌中核苷酸的提取及调味品开发研究[D].南京:南京农业大学, 2009.
[10]	邝慧聪.食用真菌中麦角甾醇及其酯的高效液相色谱分析方法与应用研究[D].广州:中山大学, 2007.
[11]	李月梅.香菇产业具有良好的发展前景[J].食品科学, 2005, 26 (7) :261-266.
[12]	Shu CH, Lin KJ.Effects of aeration rate on the production of ergosterol and blazeispirol A by Agaricus blazei in batch cultures[J].Journal of the Taiwan Institute of Chemical Engineers, 2011, 42:212-216.
[13]	T Takaku, Y Kimura, H Okuda.Isolation of an antitumor compound from Agaricus blazei Murrill and its mechanism of action[J].The Journal of Nutrition, 2001, 131:1409-141.
[14]	Hirasawa M, Shouji N, Neta T, et al.Three kinds of antibacterial substances from Lentinus edodesBerk.) Sing. (Shiitake, an edible mushroom) [J].International Journal of Antimicrobial Agents, 1999, 11:151-157.
[15]	刘延泽.植物组织破碎提取法及闪式提取器的创制与实践[J].中国天然药物, 2007, 6 (5) :401-402.
[16]	高虹, 谷文英, 丁霄霖.利用微波辅助提取测定姬松茸中麦角甾醇的含量[J].浙江大学:农业与生命科学版, 2007, 33 (1) :113-118.