卡拉胶对花生球蛋白稳定性的研究
Effects of Carrageenan on stability of arachin
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摘要: 花生蛋白饮料营养全面,风味独特,颇受消费者欢迎。而花生蛋白不稳定一直是影响花生蛋白饮料的关键问题,花生球蛋白是其主要组分,在花生蛋白稳定性中起重要作用。多糖对花生蛋白稳定性有重要的影响,然而关于阴性多糖对花生球蛋白的稳定性研究尚未见详细报道。本论文以花生球蛋白和阴离子多糖卡拉胶形成的共混体系为研究对象,以粘度、ζ-电位值、离心沉淀率等为研究指标,研究结果表明:卡拉胶对卡拉胶/花生球蛋白体系增稠效果显著;在卡拉胶浓度为0.03%0.04%时,花生球蛋白的稳定性较好。花生球蛋白浓度为1%,卡拉胶的含量为0.04%时体系稳定性最好,卡拉胶的含量继续增加反而不利于体系的稳定性。pH为7时稳定效果最佳,且温度越高越不利于体系的稳定。Na+对体系稳定性的影响较Ca2+小。Ca2+加入后导致卡拉胶与花生球蛋白发生了钙桥作用,导致稳定性降低。本实验对指导建立成熟的复配稳定剂配方工艺技术,对保证最终产品稳定性具有指导意义。Abstract: Peanut protein drinks is popular among consumers, for its variety of nutrients, especially protein, essential amina acids and unsaturated fatty acids.Peanut protein precipitation is a key issue on peanut protein drinks.As its main components, arachin palys an important role in peanut protein stability.Polysaccharides have an effect on the peanut protein stability, the studies on the stabilization mechanism of arachin induced by negative polysaccharide are still rare.According to the study on arachin and carrageenan complex system, viscosity, ζ-potential, centrifugal sedimentation rate were used to help investigate it. The study found that significant thickening effect of the carrageenan andarachin's system after adding carrageenan, the viscosity declined too.the system is stable in concentration of carrageenan from 0.03% to 0.04%.The system was the most stable when 0.04% carrageenan was added to 1% arachin solution at pH7, while excessive carrageenan was not conducive to stability.And the higher temperature was lead to instability of the system.Na+had a smaller effect on stability of system than Ca2 +.Addition of Ca2 +led to calcium bridge between arachin and carrageenan, resulting in reduced stability.It was important for guiding the establishment of the mature complex stabilizer formulation technology and ensuring the stability of the final product.