抗盐酸克伦特罗单克隆抗体的固定化及稳定性研究

王莉莉; 曹慧; 徐斐; 晏绍庆

doi:10.13386/j.issn1002-0306.2014.07.003

抗盐酸克伦特罗单克隆抗体的固定化及稳定性研究

上海理工大学医疗器械与食品学院

基金项目:

国家自然科学基金(31101283)资助；

详细信息

作者简介:
王莉莉 (1988-) , 女, 硕士研究生在读, 研究方向:食品安全与生物技术。;

中图分类号: O657.63;TS207.5
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- 文章访问数: 160
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出版历程
- 收稿日期: 2013-08-22

Immobilization and stability study of monoclonal antibody against clenbuterol

School of Medical Instrument and Engineering, University of Shanghai for Science and Technology

摘要

摘要: 采用双水相法纯化抗克伦特罗单克隆抗体(CLMcAb),并以其与酶标二抗的亲和力为指标,对CLMcAb的固定化工艺进行研究。在此基础上,通过耐冲刷实验及原子力显微镜(AFM)来表征固定化CLMcAb的稳定性。结果表明:当固定时间90min,CLMcAb稀释倍数2000倍,料液比0.65mL/颗,固定温度4℃时,固定化CLMcAb所表现出的活力最高,即OD值为1.43。纯化介质聚乙二醇(PEG)可通过在载体表面形成均匀紧密的网条结构,并降低其表面粗糙度(Ra)来提高固定化CLMcAb的稳定性。
- CLMcAb /
- 固定化 /
- 纯化 /
- 响应面 /
- 条件优化
Abstract: The anti-clenbuterol monoclonal antibody ( CLMcAb) was purified by the aqueous two-phase systems ( ATPS) , and its affinity with the second enzyme-labeled antibody was taken as evaluation indexes to optimize the immobilized process for CLMcAb. Furthermore, the stability of immobilized CLMcAb could be characterized by resist erode test and atomic force microscope ( AFM) . The results suggested that the optimum CLMcAb activity could be obtained with the fixed time of 90min, the 2000 fold dilution of CLMcAb and the ratio of solid to liquid of 0.65mL per carrier at 4℃.The immobilization of CLMcAb in PEG phase resulted in the highest activity and stability by reducing the surface roughness ( Ra) significantly and forming network micelle on the carrier surface.
- anti-clenbuterol monoclonal antibody /
- immobilization /
- purification /
- response surface methodology /
- conditions optimization

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参考文献(15)

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