Optimization of the extraction for total dietary fiber from leaves of Toona Sinensis by response surface method and its antioxidant activity
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摘要: 采用响应面法优化中性蛋白酶提取香椿叶总膳食纤维的工艺条件,并测定了其抗氧化活性。结果表明:pH5.8、酶解温度61℃、酶解时间50min、中性蛋白酶添加量0.2%,此条件下总膳食纤维得率为72.45%;香椿叶总膳食纤维浓度在210mg/mL时对DPPH·具有较强的清除能力,IC50为3.26mg/mL;对O-2·、·OH最高清除率分别为35.13%和27.38%。Abstract: Optimization of the technology for extracting total dietary fiber from leaves of Toona sinensis with neutral protease by response surface method and its antioxidant activities were studied in this paper.The results showed that the optimum conditions were pH5.8, enzymolysis temperature 61℃, time 50min and enzyme concentration0.2%.Under these conditions, the yield of total dietary fiber was 72.45%. The total dietary fiber of Toona sinensis leaves had a good scavenging capacity against DPPH radicals with IC50values 3.26mg /mL. The highest scavenging against O-2·and·OH were 35.13% and 27.38% respectively in the range of 210 mg /mL.
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[1] Schneeman B O.Dietary fiber and gastrointestinal function[J].Nutrition Research, 1998, 18 (4) :625-632.
[2] Aldoori W H, Giovannucci E L, Rockett HRH, et al.A prospective study of dietary fiber types and symptomatic diverticular disease in men[J].The Journal of Nutrition, 1998, 128 (4) :714-719.
[3] 周小理, 钱韻芳, 周一鸣, 等.植物性膳食纤维抗氧化活性的研究与应用[J].食品与机械, 2010, 26 (3) :158-160. [4] 李湘利, 刘静, 朱九滨, 等.香椿苹果复合酒的生产工艺及澄清技术研究[J].食品科技, 2011, 36 (12) :87-91. [5] Wang K J, Yang C R, Zhang Y J.Phenolic antioxidants from Chinense toon[J].Food Chemistry, 2007, 101 (1) :365-371.
[6] Miller N J, Rice Evans C A.The relative contributions of ascorbic acid and phenolic antioxidatents to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink[J].Food Chemistry, 1997, 60 (3) :331-337.
[7] 张京芳, 王冬梅, 周丽.等.香椿叶提取物不同极性部位体外抗氧化活性研究[J].中国食品学报, 2007, 7 (5) :12-17. [8] Hseu Y C, ChungWH, Chen C S, et al.Antioxidant activities of Toona sinensis leaves extracts using different antioxidant models[J].Food and Chemical Toxicology, 2008, 46 (1) :105-114.
[9] 田迪英, 杨荣.香椿的抗菌作用研究[J].食品工业科技, 2002, 23 (11) :21-22. [10] 王昌禄, 江慎华, 陈志强, 等.香椿老叶中活性物质提取及其抗氧化活性的研究[J].农业工程学报, 2007, 23 (10) :229-234. [11] 朱宏, 陈敏, 孙恬, 等.不同加工方式对香椿多酚抗氧化活性的影响[J].中国农业大学学报, 2010, 15 (4) :111-114. [12] 李燮昕, 蒲彪.锦橙皮膳食纤维抗氧化活性研究[J].食品工业科技, 2010, 31 (3) :166-168. [13] 陈钧辉, 陶力, 李俊, 等.生物化学实验[M].北京:科学出版社, 2003, 63-64. [14] 陈丛瑾, 黄克瀛, 李德良, 等.香椿叶提取物清除DPPH自由基能力的测定方法[J].林产化学与工业, 2006, 26 (3) :69-72. [15] 王光亚.保健食品功效成分检测方法[M].北京:中国轻工业出版社, 2002, 126-129. [16] 王顺民, 郑锐.正交实验优化酶法提取菜籽皮不溶性膳食纤维工艺[J].食品科学, 2013, 34 (8) :100-103. [17] 苗敬芝, 吕兆启, 曹泽虹, 等.酶法提取豆粕中总膳食纤维的研究[J].中国食品添加剂, 2009 (5) :120-124. [18] 陈永胜, 李志光, 钟慧敏, 等.葵花脱脂粕膳食纤维提取工艺的研究[J].食品科学, 2007, 28 (12) :211-215. [19] 程坷伟, 许时婴, 王璋, 等.甘薯淀粉生产废液中糖蛋白提取的超滤数学模型[J].无锡轻工大学学报, 2004, 23 (6) :59-63. [20] 张仁忠.Excel中的统计函数在医学统计中的应用[J].中国卫生统计, 2001, 18 (3) :183-185.
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