冰岛刺参岩藻糖基化硫酸软骨素降血糖及改善胰岛素抵抗的研究

田迎樱; 胡世伟; 薛长湖; 李兆杰

doi:10.13386/j.issn1002-0306.2014.05.006

冰岛刺参岩藻糖基化硫酸软骨素降血糖及改善胰岛素抵抗的研究

1. 青岛工学院食品工程学院
2. 中国海洋大学食品科学与工程学院

基金项目:

海洋公益性行业科研专项(201105029)；中国海洋大学本科生研究发展计划(OUC-SRDP)；长江学者和创新团队发展计划(IRT1188)资助；

详细信息

作者简介:
田迎樱 (1992-) , 女, 本科在读, 研究方向:食品科学与工程。;

中图分类号: R587.1
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出版历程
- 收稿日期: 2013-09-03

Study of fucosylated chondroitin sulfate from Cucumaria frondosa on hyperglycemic effects and insulin resistance improvement

College of Food Engineering, Qingdao University of Technology
College of Food Science and Engineering, Ocean University of China

摘要

摘要: 以高脂高糖饲料（high-fat high-sucrose,HFSD）饲喂法建立胰岛素抵抗小鼠模型。研究了冰岛刺参岩藻糖基化硫酸软骨素（fucosylated chondroitin sulfate from the sea cucumber Cucumaria frondosa,Cf-CHS）对胰岛素抵抗小鼠的降血糖及改善胰岛素抵抗作用。雄性C57BL/6J小鼠随机分为正常对照（标准饲料）、模型对照（HFSD）、阳性对照（HFSD+rosiglitazone（RSG）,1mg·（kg·d）-1）、Cf-CHS组（HFSD+Cf-CHS,80mg·（kg·d）-1）及Cf-CHS+RSG组（HFSD+Cf-CHS+RSG,80+1mg·（kg·d）-1）。各组小鼠自由摄食摄水19周。实验结束后,称重小鼠白色脂肪质量,检测空腹血糖、血清胰岛素及血清脂联素、抵抗素、瘦素、肿瘤坏死因子-α（TNF-α）水平。实验结果表明:Cf-CHS可显著降低胰岛素抵抗小鼠的脂肪积累（p<0.01）,降低血糖（p<0.01）和胰岛素（p<0.05）水平,改善胰岛素抵抗（p<0.05）,提高血清脂联素含量（p<0.05）,降低抵抗素（p<0.01）、瘦素（p<0.01）和TNF-α（p<0.05）含量。Cf-CHS与RSG复配使用,效果更显著（p<0.05,p<0.01）。Cf-CHS能显著改善胰岛素抵抗小鼠的高血糖症状及胰岛素抵抗程度,其作用机制可能与改善肥胖引起的脂肪细胞因子的分泌紊乱有关。
- 冰岛刺参 /
- 岩藻糖基化硫酸软骨素 /
- 血糖 /
- 胰岛素抵抗 /
- 脂肪细胞因子
Abstract: The model mice was established by feeding a diet of high-fat high-sucrose diet ( HFSD) .The aim of the present study was to unravel the effects of fucosylated chondroitin sulfate from Cucumaria frondosa ( Cf-CHS) on hyperglycemic effects and insulin resistance improvement in insulin resistant mice. Male C57BL /6 mice were randomly assigned into the normal control ( standard diet) , model control ( HFSD) , positive control ( HFSD +rosiglitazone ( RSG) , 1mg· ( kg·d) - 1) , Cf-CHS ( HFSD + + Cf-CHS, 80mg· ( kg·d) - 1) , Cf-CHS + RSG ( HFSD + Cf-CHS + RSG, 80 + 1mg· ( kg·d) - 1) groups. All animals fed with diet and water freely for 19 weeks. After experiments, the white fat weight, fasting serum glucose, serum insulin, serum adiponectin, resistin, leptin, and tumor necrosis factor- α ( TNF- α) levels were determined. Results showed that Cf- CHS markedly decreased fat deposition ( p < 0.01) , blood sugar ( p < 0.01) and insulin ( p < 0.05) levels, ameliorated insulin resistance ( p < 0.05) , increased serum adiponectin concentration ( p < 0.05) , and lowered serum resistin ( p < 0.01) , leptin ( p < 0.01) , TNF-α ( p < 0.05) levels.The effect was more significant when Cf- CHS compounded with RSG ( p < 0.05, p < 0.01) .Cf-CHS significantly decreased blood glucose level and ameliorated insulin resistance in insulin resistant mice, and the mechanisms may involved the regulation of adipocytokines parasecretion caused by adiposity.
- Cucumaria frondosa /
- fucosylated chondroitin sulfate from sea cucumber /
- blood glucose /
- insulin resistance /
- adipocytokines

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