GPC/SEC与激光光散射联用技术在食物蛋白分析中的应用研究进展

肖娜; 佟平; 熊丽姬; 陈红兵; 高金燕

doi:10.13386/j.issn1002-0306.2014.04.022

GPC/SEC与激光光散射联用技术在食物蛋白分析中的应用研究进展

肖娜^1,2,
佟平¹,
熊丽姬^1,3,
陈红兵^1,3,
高金燕²

1. 南昌大学食品科学与技术国家重点实验室
2. 南昌大学生命科学与食品工程学院
3. 南昌大学中德联合研究院

基金项目:

国家高技术研究发展计划(863计划)(2013AA102205)；国家国际科技合作专项(2013DFG31380)；国家自然科学基金(21162019)；江西省科技计划项目(20122BBG70170-2)；

详细信息

作者简介:
肖娜 (1991-) , 女, 硕士研究生, 研究方向:食品生物技术。;

中图分类号: TS207.3;O657.3
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出版历程
- 收稿日期: 2013-07-07

Application of GPC/SEC coupled with laser light scattering on food proteins

State Key Laboratory of Food Science and Technology, Nanchang University
College of Life Sciences and Food Engineering, Nanchang University
Sino-German Joint Research Institute, Nanchang University

摘要

摘要: GPC/SEC与(LLS)联用技术在分离和测定大分子物质的分子量及其分布中发挥着越来越重要的作用。该方法所需样品少、快速、准确,而且可以同时测定出物质的重均分子量(Mw)、数均分子量(Mn)、Z均分子量(Mz)、Mw/Mn、第二维里系数(A2)以及均方根半径(Rg)。目前GPC/SEC与激光光散射联用技术主要用于测定蛋白质的分子量及其分布以及研究蛋白质的聚合状态,显示了其与众不同的性能,而且该技术在食物蛋白中的应用仍值得进一步研究。
- GPC/SEC /
- 激光光散射 /
- 分子量 /
- 食物蛋白
Abstract: GPC/SEC and laser light scattering (LLS) combined technology play a more and more important role in the separation determination of molecular weight and its distribution of macromolecular substances. This method requires less samples, and also was fast and accurate, moreover it could also determine average molecular weight (Mw) , the number average molecular weight (Mn) , Z-average molecular weight (Mz) , Mw /Mn, the second virial coefficient (A2) and root mean square radius (Rg) of the material. GPC/SEC and laser light scattering detection application in protein was summarized. At present, the technology mainly used for the determination of protein molecular weight and its distribution as well as the study of protein aggregation state, and showed its distinctive performance, however, its application in food protein deserved further study.
- GPC/SEC /
- laser light scattering /
- molecular weight /
- food proteins

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