Preparation of antioxidant peptides from Zizyphi Spinosi Semen protein by enzymatic hydrolysis
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摘要: 为优化Alcalase蛋白酶酶解酸枣仁蛋白制备抗氧化肽的工艺条件,以DPPH自由基清除率作为指标,通过单因素试验考察酶解温度、底物浓度、酶与底物浓度比（[E]/[S]）、酶解pH和时间对DPPH自由基清除率的影响,在单因素试验的基础上,采用Box-Behnken设计,建立并分析各因素与清除率之间关系的数学模型;并对酸枣仁蛋白酶解产物的氨基酸组成及含量进行分析。结果表明:酶解酸枣仁蛋白制备抗氧化肽的最佳工艺条件为酶解温度51.2℃、酶解pH8.1、底物浓度4.7%,在此条件下DPPH自由基清除率的最大理论值为91.59%,该回归模型具有较好的预测性能,可用于指导生产实践。酸枣仁蛋白酶解产物共含有17种氨基酸,富含脯氨酸、甘氨酸和谷氨酸。Abstract: Optimization enzymolysis technology for production of antioxidant peptides of Zizyphi Spinosi Semen protein were studied. The antioxidant activities of enzymatic hydrolysates from spine date seed protein were investigated by 1, 1- diphenyl- 2- picrylhydrazyl ( DPPH) radical scavenging activities. Effect of enzymolysis temperature, substrate concentration, ratio of enzyme to substrate ( [E]/[S]) , enzymolysis pH and hydrolysis time on the DPPH scavenging rate of enzymatic hydrolysate from spine date seed protein were taken by the single factor experiments. Technology parameters were optimized by Box- Behnken statistical design. The amino acid composition and content of enzymatic hydrolysate of Zizyphi Spinosi Semen protein was measured by protein sequencer.The results showed that when the extraction parameters were controlled at enzymolysis temperature 51.2℃, enzymolysis pH8.1, substrate concentration 4.7%, the DPPH scavenging rate could be up to 91.59%. The predictive models are feasible in practice.Enzymatic hydrolysate of Zizyphi Spinosi Semen protein, consisting of 17 kinds of amino acid, was rich in proline, glycine and glutamic acid.
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[1] 李续娥, 李维凤, 裴渭静.酸枣仁与缅枣仁的蛋白质分析[J].中草药, 2002, 33 (1) :26-28. [2] Ma Y, Han H, Eun J S, et al.Sanjoinine A isolated from Zizyphi Spinosi Semen augments pentobarbital-induced sleeping behaviors through the modification of GABA-ergic systems[J].Biol Pharm Bull, 2007, 30 (9) :1748-1753.
[3] Yoon S R, Jo Y J, Yang S, et al.Sanjoinine A isolated from Semen Zizyphi Spinosi protects against kainic acid-induced convulsions[J].Arch Pharm Res, 2009, 32 (11) :1515-1523.
[4] Matsuda H, Murakami T, Ikebata A, et al.Bioactive saponins and glycosides.XIV.Structure elucidation and immunological adjuvant activity of novel protojujubogenin type triterpene bisdesmosides, protojujubosides A, B, and B1, from the seeds of Zizyphus jujuba var.spinosa (Zizyphi Spinosi Semen) [J].Chem Pharm Bull, 1999, 47 (12) :1744-1748.
[5] 丁轲, 谷禹, 陆晶晶, 等.酸枣仁黄酮组分提取纯化工艺的研究[J].中国食品学报, 2011, 11 (4) :62-70. [6] 张君慧, 张晖, 王兴国, 等.抗氧化活性肽的研究进展[J].中国粮油学报, 2008, 23 (6) :228-231. [7] 陈美珍, 余杰, 郭慧敏.大豆分离蛋白酶解物清除自由基作用研究[J].食品科学, 2002, 23 (1) :43-47. [8] 张强, 阚国仕, 陈红漫.酶解玉米蛋白粉制备抗氧化肽[J].食品工业科技, 2005, 26 (6) :109-111. [9] Wang J S, Zhao M M, Zhao Q Z, et al.Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems[J].Food Chemistry, 2007, 101 (4) :1658-1663.
[10] Oyaizu M.Studies on products of browning reactions antioxidative activities of products of browning reaction prepared from glucosamine[J].Japanese Joumal of Nutrition, 1986, 44:307-315.
[11] 康玮丽, 唐军虎, 敬思群.酶解核桃蛋白制备抗氧化肽工艺条件优化[J].食品与发酵工业, 2010, 36 (12) :94-99. [12] Rajapakse N, Mendis E, Byun H G, et al.Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems[J].J Nutr Biochem, 2005, 16 (9) :562-569.
[13] CHEN H M, Muramoto K, Yamauchi F, et al.Antioxidant activity of designed peptides based on the antioxidative peptide isolated from digests of a soybean protein[J].J Agric Food Chem, 1996, 44 (9) :2619-2623.
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