琯溪蜜柚果汁树脂吸附脱苦前后酚类物质含量的测定

钟晓婷; 嵇海峰; 许键; 黄高凌

doi:10.13386/j.issn1002-0306.2014.02.081

琯溪蜜柚果汁树脂吸附脱苦前后酚类物质含量的测定

1. 集美大学生物工程学院
2. 福建省高校食品微生物与酶工程技术研究中心
3. 厦门市食品与生物工程技术研究中心

基金项目:

国家自然科学基金(31271914)；福建省教育厅项目(JA12188)；

详细信息

作者简介:
钟晓婷 (1988-) , 女, 硕士研究生, 研究方向:食品化学。;

中图分类号: TS255.44
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出版历程
- 收稿日期: 2013-07-04

Determination for phenolic compounds of fresh and resin adsorption debittered Guanxi pomelo juice

College of Bioengineering, Jimei University
Food Microorganism and Enzyme Engineering Technology Research Center of Fujian University
The Research Center of Food Biotechnology

摘要

摘要: 利用高效液相色谱法测定了琯溪蜜柚果汁酚类成分的含量,并研究了吸附脱苦对琯溪蜜柚果汁中酚类物质含量的影响。结果表明,琯溪蜜柚果汁中含有绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质;用高效液相色谱法分析琯溪蜜柚果汁中酚类物质,它们的加标回收率均在85.0%¹03.6%之间,相对标准偏差（RSD）均在0.48%².9%之间。经XAD-16树脂吸附脱苦后,柚汁中的香豆酸、阿魏酸、芸香苷等三种酚类成分已检测不到,而绿原酸、柚皮苷和咖啡酸的损失率分别为97.2%、93.5%、87.9%,说明树脂吸附脱苦导致琯溪蜜柚汁中绿原酸、咖啡酸、香豆酸、阿魏酸、芸香苷和柚皮苷等六种酚类物质含量严重降低。
- 琯溪蜜柚果汁 /
- 吸附脱苦 /
- 酚类物质 /
- 高效液相色谱法
Abstract: The effect of adsorption debittering on phenolic compounds of Guanxi pomelo juice were studied. A HPLC method had been developed to determinate concentration changes in phenolic compounds of the pomelo juice after the Amberlite XAD-16 resin adsorption. The result showed that the pomelo juice contained six phenolic compounds, namely chlorogenic acid, caffeine acid, p-coumaric, ferulic acid, ruin, naringin. The HPLC method had recoveries to these phenolic compounds in a range within 85.0% to 103.6%, and the relative standard deviations was in a range within 0.48%².9%. After resin adsorption debittering treatment, p-coumaric, ferulic acid, ruin could not be detected, and the content of chlorogenic acid, naringin, caffeine acid decreased 97.2%, 93.5%, 87.9% of pomelo juice, respectively. These results indicate that the resin adsorption debittering dramatically reduce the content of chlorogenic acid, caffeine acid, p-coumaric, ferulic acid, ruin, naringin in the pomelo juice.
- Guanxi pomelo juice /
- adsorption debittering /
- phenolic compounds /
- high performance liquid chromatograph

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