高效液相色谱法测定芒果皮中脱落酸的含量

杨杨; 范蓓; 生吉萍; 申琳

doi:10.13386/j.issn1002-0306.2014.02.016

高效液相色谱法测定芒果皮中脱落酸的含量

杨杨^1,2,
范蓓³,
生吉萍⁴,
申琳¹

1. 中国农业大学食品科学与营养工程学院
2. 内蒙古农业大学食品科学与工程学院
3. 农业部农产品加工综合性重点实验室,中国农业科学院农产品加工研究所
4. 中国人民大学农业与农村发展学院

基金项目:

中国农业科学院基本科研业务费(0032012018)；

详细信息

作者简介:
杨杨 (1981-) , 女, 在读博士, 讲师, 研究方向:果蔬采后生理与生物技术。;

中图分类号: S667.7
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出版历程
- 收稿日期: 2013-04-14

Analysis of abscisic acid content in peel of mango by HPLC

Key Laboratory of Agro-Products Processing, MOA, Institute of Agro-products Pprocessing Science and Technology, Chinese Academy of Agricultural Sciences
College of Food Science and Engineering, Inner Mongolia Agricultural University
College of Food Science and Nutritional Engineering, China Agricultural University
School of Agricultural Economics and Rural Development, Renmin University of China

摘要

摘要: 建立了高效液相色谱（HPLC）测定芒果果皮中脱落酸（ABA）含量的方法。确定了以无水乙醚为提取试剂,0.5%碳酸氢钠、乙酸乙酯和氯仿为萃取试剂的前处理过程,得到色谱条件为ODS-C18反相柱（4.6mm×250mm,5μm）,以甲醇和pH3.0乙酸溶液（60∶40,v/v）作为流动相,流速为0.8mL/min,检测器波长为252nm进行检测。结果表明,该方法标准曲线在0.50¹0.0mg/L范围内线性关系良好,R2=0.9996,平均回收率95.90%。该方法简单快速,为芒果采后ABA的生理作用研究提供了重要的技术支持。
- 高效液相色谱 /
- 芒果皮 /
- 脱落酸
Abstract: A HPLC method for determining abscisic acid (ABA) in peel of mango was established. The samples were homogenized in diethyl ether, partitioned by sodium carbonate solution, ethyl acetate and chloroform, separated on ODS-C18 column (4.6mm×250mm, 5μm) , eluted with the mixture of methanol and acetic acid solution at pH=3.0 (60∶40, v/v) at 0.8mL/min, and detected at 252nm. Results showed that the calibration curves of ABA had good linearity over the range from 0.50 to 10.0mg/L with a correlation coefficient of 0.9996. The average recovery rate for ABA was 95.90%. This method was simple and fast, which will provide technical support for the research of physiology function of ABA.
- HPLC /
- peel of mango /
- ABA

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参考文献(10)

[1]	Policegoudra RS, Aradhy SM.Biochemical changes and antioxidant activity of mango ginger (Curcuma amada Roxb.) rhizomes during postharvest storage at different temperatures[J].Postharvest Biology and Technology, 2007, 46:189-194.
[2]	Sugunya C, Saichol K, Judith B, et al.Chilling injury in mango fruit peel:Cultivar differences are related to the activity of phenylalanine ammonia lyase[J].Postharvest Biology and Technology, 2011, 62:59-63.
[3]	朱世江, 季作梁.热处理提高芒果抗冷性与内源ABA的关系[J].中国农业科学, 2002, 35 (9) :1150-1153.
[4]	丁静, 沈镇德, 方亦雄, 等.植物内源激素的提取分离和生物鉴定[J].植物生理学通讯, 1979 (2) :27-39.
[5]	柴孟颖, 李秀根, 张绍铃.梨幼果中脱落酸和吲哚-3-乙酸的简易气相色谱分析[J].果树学报, 2005, 22 (3) :276-278.
[6]	李金克, 陈华君, 陈少良.GC-MS内标法定量分析植物组织中的JA、IAA和ABA[J].北京林业大学学报, 2010, 32 (9) :143-147.
[7]	李宗霆.植物激素及其免疫检测技术[M].南京:南京科学技术出版社, 1996.
[8]	丁杰, 秦献军, 张耀武.用高效液相色谱仪测定葡萄休眠芽的脱落酸含量[J].落叶果树, 2007 (3) :4-6.
[9]	Andrew RSR, Stephen JA, Adrian JC.Determination of endogenous and supplied denudated abscisic acid in plant tissues by high-performance liquid chromatography electrospray ionization tandem mass spectrometrywithmu tiple reaction monitoring[J].Analytical Biochemistry, 2004, 329:324-333.
[10]	曾成鸣, 叶运奎, 严怡怡, 等.反相高效液相色谱法测定水果中顺式脱落酸含量[J].化学研究与应用, 1992 (4) :66-68.