Abstract: A HPLC method for determining abscisic acid (ABA) in peel of mango was established. The samples were homogenized in diethyl ether, partitioned by sodium carbonate solution, ethyl acetate and chloroform, separated on ODS-C18 column (4.6mm×250mm, 5μm) , eluted with the mixture of methanol and acetic acid solution at pH=3.0 (60∶40, v/v) at 0.8mL/min, and detected at 252nm. Results showed that the calibration curves of ABA had good linearity over the range from 0.50 to 10.0mg/L with a correlation coefficient of 0.9996. The average recovery rate for ABA was 95.90%. This method was simple and fast, which will provide technical support for the research of physiology function of ABA.