Abstract: Taking fruit of Rose Laevigata Michx. as material, the yield of falconoid indicators as the evaluation standard, and its flavonoid extraction process with traditional extraction and microwave- assisted extraction were optimized by single factor and orthogonal experiment. Validation experiments showed that the flavonoid yield of traditional methods was 7.84% with the RSD 0.89%, and that yield of microwave-assisted methods was 9.06% with the RSD 1.01%.Microwave technique to extract flavonoids from the fruit of Rose Laevigata Michx.not only reduced extraction time but also improved experimental efficiency.Therefore, the flavonoid extraction could be improved by the appropriate conditions.