Abstract: The object of this research was to optimize ultrasonic- assisted extraction process of chlorogenic acid from Lonicera macranthoides Hand.-Mazz.The chlorogenic acid was tested by HPLC.To maximize the extraction of chlorogenic acid, main technological parameters like ultrasonic extraction time, ethanol concentration, solid /liquid ratio and temperature, were investigated by single factor method coupled with L 9 ( 34) orthogonal array design. Results showed that the optimal conditions of extracting chlorogenic acid from Lonicera macranthoides was 50% ethanol, ultrasonic power 300W, ultrasonic frequency 59kHz, extracting 50min, with a solid /liquid ratio of 1∶50.Under above conditions, the extraction yield of chlorogenic acid reached 88.7624 mg /g.It was proved that this extracting technology was reasonable and feasible.The results demonstrated that extract from Lonicera macranthoides had certain reduction capacity and potent DPPH radical scavenging capacity and could be used as antioxidants and in functional foods.