蚕蛹蛋白和蚕蛹短肽的模拟胃肠道消化特性研究

陈佳捷; 吴文惠; 倪玲; 严婷; 赵清波; 包斌

doi:10.13386/j.issn1002-0306.2014.01.004

蚕蛹蛋白和蚕蛹短肽的模拟胃肠道消化特性研究

陈佳捷¹,
吴文惠^2,3,
倪玲¹,
严婷¹,
赵清波¹,
包斌^1,3

1. 上海海洋大学食品学院
2. 上海海洋大学水产品加工与贮藏工程研究中心海洋科学研究室
3. 上海海洋大学海洋科学研究室

基金项目:

上海市自然科学基金(10ZR1413800)； 863项目(2011AA09070109)；上海市科委工程中心建设(11DZ2280300)；

详细信息

作者简介:
陈佳捷 (1988-) , 女, 硕士研究生, 研究方向:食品科学与工程专业。;

中图分类号: R151
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出版历程
- 收稿日期: 2013-06-12

Nutritional characteristics of silkworm pupae protein and peptides after simulated gastric and simulated intestinal digestion in vitro

College of Food Science and Technology, Shanghai Ocean University
Shanghai Engineering Research Center of Aquatic Product Processing & Preservation, Institutes of Marine Science
Shanghai Ocean University, Institutes of Marine Science

摘要

摘要: 研究了蚕蛹蛋白和蚕蛹短肽的氨基酸组成,并分析了其在模拟胃肠道消化过程中的消化特性和氮释放量,探究蚕蛹蛋白和短肽在人类营养支持方面的应用。使用自动氨基酸分析仪测定蚕蛹蛋白和蚕蛹短肽的氨基酸含量,并计算氨基酸评分(AAS)、化学评分(CS)和必需氨基酸指数(EAAI)。蚕蛹蛋白和蚕蛹短肽的消化特性和氮释放量通过体外模拟胃肠道消化实验和凯氏定氮法进行评价的计算。结果表明,蚕蛹蛋白和蚕蛹短肽含有人体所需的18种氨基酸,而且必需氨基酸的组成合理。蚕蛹蛋白和蚕蛹短肽在体外模拟胃肠道消化实验中有良好高效的消化率和较高的氮释放量(约90%)。因此在今后将蚕蛹蛋白和蚕蛹短肽作为优质且经济的蛋白质原料,在动物饲料和人类肠内营养支持制剂方面有巨大的应用潜力。
- 蚕蛹蛋白和短肽 /
- 氨基酸分析 /
- 模拟胃肠道消化 /
- 氮释放量
Abstract: This study was designed to evaluate the nutritional characteristics and percentage of released nitrogen ( %) of silkworm pupae proteins and peptides after simulated gastric and intestinal digestion, with a view to use in animal feeds or human nutritional support.Distribution of amino acids in the processed protein was analyzed and the amino acid score, chemical score, and essential amino acid index ( EAAI) were calculated. The method of simulated gastrointestinal digestion in vitro and Kjeldahl determination were used to analyze absorption characteristics and the released nitrogen ( %) of samples. Silkworm pupae proteins and peptides contained all amino acids required by humans, and the composition was reasonable. Simulated gastrointestinal digestion resulted in about 90% nitrogen release amount. The data indicated that this protein might be useful as an economic, high quality supplement in animal feeds and for human nutritional support.
- silkworm pupae proteins and peptides /
- amino acid analysis /
- simulated gastrointestinal digestion /
- released nitrogen

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