反相高效液相色谱法测定秋葵干蔬中的β-胡萝卜素

董爱军; 杨鑫; 张英春; 张华; 井晶

doi:10.13386/j.issn1002-0306.2013.24.027

反相高效液相色谱法测定秋葵干蔬中的β-胡萝卜素

哈尔滨工业大学食品科学与工程学院

详细信息

作者简介:
董爱军 (1969-) , 女, 硕士, 副教授, 研究方向:食品质量与安全。;

中图分类号: TS255.7;O657.72
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出版历程
- 收稿日期: 2013-05-15

Determination of β-carotene in dry okra by reverse phase high performance liquid chromatography

College of Food Science and Engineering, Harbin Institute of Technology

摘要

摘要: 建立了测定秋葵干蔬中β-胡萝卜素的高效液相色谱法。样品皂化后,采用反相高效液相色谱-紫外可见检测器进行测定,外标法定量。色谱柱为C18柱（250mm×4.6mm,i.d.5μm）,流动相为乙腈-甲醇-乙酸乙酯（88∶10∶2）,流速1mL/min,检测波长453nm。结果表明,β-胡萝卜素在0.1¹0.0mg/L浓度范围内呈现良好的线性关系,相关系数为0.996;β-胡萝卜素的回收率为82.4%⁹4.0%,相对标准偏差（RSD）为2.28%⁵.43%;秋葵干蔬中β-胡萝卜素的检出限（LOD）为0.08mg/kg,定量下限（LOQ）为0.26mg/kg。本方法的准确度和精密度都很高,能够满足实际测定需要。
- 反相高效液相色谱 /
- β-胡萝卜素 /
- 秋葵干蔬
Abstract: Okra was a popular health food in the world, and it could be used for many purposes, such as a vegetable, soup ingredient and fat substitute in chocolate.In this paper, a method was developed for the determination of β-carotene in dried okra by reverse phase high performance liquid chromatography (RP- HPLC) .After the sample was extracted with ethanol, the mixture was saponified at room temperature overnight, and then the saponification liquid was extracted with n-hexane.The extract was separated on a C18 column (250mm×4.6mm, 5μm) at 30℃, which was eluted with acetonitrile-methyl alcohol-ethyl acetate (88∶10∶2, v∶v∶v) at a rate of 1mL/min, and the signal was detected at 453nm.The results showed that:β-carotene was linear over the concentration range of 0.1¹0.0mg/L with a correlation coefficient of 0.996;the recovery of β-carotene in dried okra was 82.4%⁹4.0%, the relative standard deviations (RSD) was 2.28%⁵.43%, the limit of detection (LOD) of β-carotene in dried okra was 0.08mg/kg, the limit of quantification (LOQ) was 0.26mg/kg.This method was reliable and accurate, and it could meet the demands of actual measurement.
- RP-HPLC /
- β-carotene /
- dried okra

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