The preparation of bioactive peptides by fermentation and the hydrolyzates determination
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摘要: 以4株蛋白水解能力较强的瑞士乳杆菌作为出发菌株,分别测定其36h内的蛋白水解动力曲线、产酸曲线,经比较分析选出一株产肽能力强、速率快的瑞士乳杆菌作为后续实验用株。采用响应面分析法优化发酵条件,TricineSDS-PAGE电泳法对发酵产物进行初步分析。结果表明:所选瑞士乳杆菌在接种量为3.5%,底物浓度13%,发酵温度37℃,发酵时间18h的条件下,水解度可达5.60%,可将乳蛋白水解成大量的生物活性肽,其中包括很多小分子肽段,表明该菌可作为水解乳蛋白制备生物活性肽的优良菌株。
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关键词:
- 乳酸菌 /
- pH /
- 水解度 /
- 响应面分析 /
- Tricine-SDS-PAGE电泳法
Abstract: Four lactobacillus helveticus with high proteolytic activity were regarded as the research subject, the changes in degree of hydrolysis (DH) and pH were examined periodically during 36 hours of fermentation.One of strains was selected for further study.Fermentation condition was optimized by response surface analysis and the hydrolyzates were determined by SDS- PAGE.The results showed that optimum hydrolysis condition was as follows:inoculum size of 3.5%, substrate concentration of 13%, hydrolysis temperature of 37.0℃, fermentation time of 18h.The degree of hydrolysis was 5.60% and the hydrolyzates contained a large number of small peptide fragments.So the strain was an optimal strain for the production of small peptides derived from fermented milk. -
[1] Fitzgerald G F, Murray J A.Bioactive peptides and lactic fermentations[J].International Journal of Dairy Technology, 2006, 59 (2) :118-112.
[2] Shrikant S, Singh S, Shashank R.Bioactive Peptides:A Review[J].Int J Bioa-Utomation, 2011, 15 (4) :223-250.
[3] Juillard V, Laan H, Kunj ERS, et al.The extracellular PI-type proteinase of Lactococcus lactis hydrolyzes beta-casein into more than one hundred different oligopeptides[J].Journal of Bacteriology, 1995, 177 (12) :3472-3478.
[4] Muguerza B, Ramos M, Sánchez E, et al.Antihypertensive activity of milk fermented by Enterococcusfaecalis strains isolated from raw milk[J].International Dairy Journal, 2006, 16 (1) :61-69.
[5] Ueno K, Mizuno S, Yamamoto N.Purification and characterization of an endopeptidase that has an important role in the carboxyl terminal processing of antihypertensive peptides in Lactobacillushelveticus CM4[J].Letters in Applied Microbiology, 2004, 39 (4) :313-318.
[6] Pirainoa P, Zotta T.Acid production, proteolysis, autolytic and inhibitory properties of lactic acid bacteria isolated from pasta filata cheeses:A multivariate screening study[J].International Dairy Journal, 2008, 18 (1) :81-92.
[7] 赵新淮, 冯志彪.蛋白质水解物水解度的测定[J].食品科学, 1994 (11) :65-67. [8] 孙懿琳, 田辉, 崔兰, 等.保加利亚乳杆菌生长性能与后酸化的关系初探[J].食品科技, 2012, 37 (10) :7-12. [9] 孙辉, 赵新淮.酪蛋白水解物的类蛋白反应修饰及其产物ACE抑制活性特征[J].食品科学, 2011, 32 (19) :60-65. [10] 殷捷, 卢姗姗, 张少辉, 等.不同瑞士乳杆菌发酵牛乳产肽的比较研究[J].食品科学, 2010, 31 (15) :186-189.
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