微波辅助提取枇杷花中齐墩果酸和熊果酸的工艺优化

汪文浩; 郑美瑜; 陆胜民

doi:10.13386/j.issn1002-0306.2013.22.042

微波辅助提取枇杷花中齐墩果酸和熊果酸的工艺优化

南京农业大学食品科技学院
浙江省果蔬保鲜与加工技术研究重点实验室, 浙江省农业科学院食品科学研究所

基金项目:

浙江省植物食品加工技术重点科技创新团队项目(2010R50032)；

详细信息

作者简介:
汪文浩 (1988-) , 男, 硕士研究生, 研究方向:农产品加工与贮藏工程。;

中图分类号: TQ914
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出版历程
- 收稿日期: 2013-04-26

Optimization of microwave-assisted extracting oleanolic acid and ursolic acid from loquat flowers

College of Food Science and Technology, Nanjing Agricultural University
Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences

摘要

摘要: 研究枇杷花中齐墩果酸和熊果酸的微波辅助提取最佳工艺条件,为合理利用枇杷花资源提供理论基础和技术依据。采用单因素及L9(34)正交实验筛选最佳提取条件,并对不同的提取方法进行比较;采用高效液相色谱法同时测定齐墩果酸和熊果酸的含量。结果:最佳提取工艺为乙醇体积分数80%、液固比40∶1mL/g、提取时间50min、微波功率300W、提取温度50℃,齐墩果酸和熊果酸提取量分别达到0.482、3.118mg/g,均显著高于超声波辅助提取和热回流提取的提取量(p<0.01)。
- 枇杷花 /
- 齐墩果酸 /
- 熊果酸 /
- 微波辅助提取
Abstract: This paper aimed at optimizing these conditions of microwave-assisted extracting oleanolic acid (OA) and ursolic acid (UA) from loquat (Eriobotyra japonica) flowers to provide theoretical and technical support for the rational utilization of the flower resources. Single-factor and orthogonal experiments were applied to determine the optimal microwave-assisted extraction conditions and its effectiveness was compared with the other extraction methods. Contents of OA and UA were determined simultaneously by HPLC. Results indicated that the extraction yields of OA and UA were 0.482mg/g dry weight (DW) and 3.118mg/g DW respectively under the optimal microwave-assisted extraction conditions:80% of ethanol concentration, 40∶1 in liquid/solid ratio (mL/g) , 50min in extraction time, 300W for microwave power and extraction at 50℃. Extraction yields of OA and UA by microwave-assisted method were significantly higher than those using ultrasonic-wave assisted extraction and thermo-refluxing extraction techniques.
- loquat (Eriobotyra japonica) flowers /
- oleanolic acid /
- ursolic acid /
- microwave-assisted extraction

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