蝉花多糖提取工艺优化研究

鲁吉珂; 古国峰; 汪瑞; 胡甜; 郝利民; 余坚勇; 王英泽

doi:10.13386/j.issn1002-0306.2013.22.040

蝉花多糖提取工艺优化研究

1. 郑州大学生命科学学院
2. 总后军需装备研究所军用汉麻材料研究中心

基金项目:

全军重点科研项目(AX110C002)；总后给养勤务科研项目；

详细信息

作者简介:
鲁吉珂 (1982-) , 男, 博士, 讲师, 研究方向:食品生物技术。;

中图分类号: TQ914
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出版历程
- 收稿日期: 2013-08-15

Optimization of extraction of polysaccharide from Cordyceps sobolifera

School of Life Sciences, Zhengzhou University
The Research Center of China-Hemp Materials, The Quartermaster Equipment Institute of General Logistics Departmentof People's Liberation Army

摘要

摘要: 以浙江蝉花为原料,建立了多糖提取率的计算方法,并对蝉花多糖的提取工艺进行优化。采用水为溶剂,以提取温度、时间、料液比和提取次数为考察因素,在单因素优化的基础上设计4因素4水平正交实验,研究蝉花多糖最优提取条件。结果表明,各因素对蝉花多糖提取率影响顺序依次为:提取温度>提取次数>料液比>提取时间。最优提取条件为:温度90℃,料液比1∶40(g∶mL),提取240min,连续提取4次。在此条件下,蝉花多糖提取率为11.82%。
- 蝉花 /
- 多糖 /
- 水 /
- 浸提 /
- 优化
Abstract: Extraction of polysaccharide from Cordyceps sobolifera in Zhejiang was investigated, and the calculation method of the extraction yield was determined. With water as extraction solvent, the effect of extraction temperature, extraction time, solid-liquid ratio and extraction times on the yield of polysaccharide were optimized with single factor test followed by four factor-four level orthogonal experiment. The result showed that the effect of the investigated factors on the polysaccharide extraction yield was in the order as extraction temperature>extraction times>solid-liquid ratio>extraction time. The optimal extraction conditions of polysaccharide were that extraction temperature of 90℃, solid-liquid ratio of 1∶40 (g∶mL) , extraction time of240min and extraction 4 times. Under these conditions the polysaccharide extraction yield was 11.82%.
- Cordyceps sobolifera /
- polysaccharide /
- water /
- extraction /
- optimization

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参考文献(15)

[1]	许胜杰, 叶淑幸, 陈劲初.新资源食品蝉花菌丝体的开发[J].食药用菌, 2011, 19 (6) :34-38.
[2]	温鲁, 唐玉玲, 张平.蝉花与有关虫草活性成分检测比较[J].江苏中医药, 2006, 27 (1) :45-46.
[3]	官宗华, 宋玉良.蝉花的研究概况[J].中国中药指南, 2012, 10 (10) :443-444.
[4]	Weng S-C, Chou C-J, Lin L-C, et al.Immunomodulatory functions of extracts from the Chinese medicinal fungus Cordyceps cicadae[J].Journal of Ethnopharmacology, 2002, 83 (1-2) :79-85.
[5]	Wu M-F, Li P-C, Chen C-C, et al.Cordyceps sobolifera extract ameliorates lipopolysaccharide-induced renal dysfunction in the rat[J].The American Journal of Chinese Medicine, 2011, 39 (3) :523-535.
[6]	俞滢, 顾蕾, 陈启琪, 等.蝉花的化学成分分析[J].杭州师范学院学报, 1997 (6) :61-63.
[7]	余佳文.蝉花中多球壳菌素类长链碱的测定方法学研究及代谢轮廓分析[D].重庆:重庆大学, 2009.
[8]	Wang S-X, Liu Y, Zhang G-Q, et al.Cordysobin, a novel alkaline serine protease with HIV-1 reverse transcriptase inhibitory activity from the medicinal mushroom Cordyceps sobolifera[J].Journal of Bioscience and Bioengineering, 2012, 113 (1) :42-47.
[9]	徐晓飞, 陈健.多糖含量测定的研究进展[J].食品科学, 2009, 30 (21) :443-448.
[10]	何晓波, 芦柏震, 周俐斐, 等.蝉花总多糖对细胞免疫和体液免疫反应的促进作用[J].中华中医药学刊, 2010, 28 (7) :1465-1468.
[11]	朱燕, 程东庆.蝉花多糖抗氧化活性的测定[J].中华中医药学刊, 2009, 27 (7) :1552-1554.
[12]	姚婷, 罗靖, 宋捷民.金蝉花多糖的微波辅助提取工艺研究[J].江西中医学院学报, 2012, 24 (5) :73-76.
[13]	余文娟, 许明敏, 潘丽辉, 等.蝉花多糖超声波辅助提取工艺的优化[J].湖北农业科学, 2012, 51 (20) :4598-4600.
[14]	周俐斐, 芦柏震, 蔡菊芬.蝉花总多糖的提取纯化及含量测定[J].江西中医药, 2009, 40 (1) :56-57.
[15]	郝利民, 张建春, 鲁吉珂, 等.云南种植玛咖中多糖提取工艺的优化研究[J].食品工业科技, 2013 (3) :262-264.