响应面分析法对α-淀粉酶抑制剂筛选方法的优化

基金项目:

广西基金资助项目(桂科基03420034)；

详细信息

Optimization of the method for screening of α-amylase inhibitors by response surface methodology

摘要: 采用工业α-淀粉酶代替胰α-淀粉酶,利用响应面分析法优化α-淀粉酶抑制剂的筛选方法。采用单因素法、响应面分析法对α-淀粉酶反应体系的pH、孵化温度、可溶性淀粉浓度与α-淀粉酶浓度比例等分别进行考察和优化。用3,5-二硝基水杨酸法（Bemfeld法）测定α-淀粉酶活性。最终确定的体系反应条件为:pH6.6、温度55℃、比例为1∶1.5。结果表明,优化后的α-淀粉酶抑制剂筛选方法稳定可靠、重现性好,可用于α-淀粉酶抑制剂的筛选。
- α-淀粉酶抑制剂 /
- 响应面分析法 /
- 糖尿病
Abstract: The purpose of this study was to optimize α-amylase inhibitors by response surface methodology with industrial α-amylase instead of pancreatic α-amylase and to investigate α-amylase reactive system included pH value, incubation temperature, and proportion in soluble amylum and α-amylase by single factor analysis and response surface analysis. The α-amylase activity was examed by 3, 5-dinitrosalicylic acid colorimetric assays (Bemfeld method) . The final conditions of reactive system were pH6.6 value, 55℃ and proportion of 1∶1.5. Results showed that the optimize method of α-amylase inhibitors was stable, reliable and well repeated. It could be used to screen the inhibitors of α-amylase.
- α-amylase inhibitors /
- response surface methodology /
- diabetes

[1]	陈浙江, 袁萍, 叶晓平, 等.治疗糖尿病常用中药对α-葡萄糖苷酶和α-淀粉酶的抑制活性研究[J].中成药, 2008 (11) :1661-1664.
[2]	赵蓉, 李多伟, 沈晓东, 等.白芸豆中α-淀粉酶抑制剂的研究[J].中成药, 2008, 30 (9) :3.
[3]	Sharma A, Gupta M.Three phase partitioning as a largescale separation method for purification of a wheat germ bifunctional protease/amylase inhibitor[J].Process Biochemistry, 2001, 37 (2) :193-196.
[4]	赵蓉, 李多伟, 任涛, 等.Dns比色法测定白芸豆中α-淀粉酶抑制剂活性的方法研究[J].中成药, 2013 (3) :573-576.
[5]	贾光锋.Α-淀粉酶抑制剂的制备及检测[J].食品与药品, 2007 (2) :34-36.
[6]	赵修南, 贾启燕, 单俊杰.Α-淀粉酶抑制剂筛选方法的优化[J].国际药学研究杂志, 2008, 35 (5) :4.
[7]	魏锐, 田惠玲, 周建军, 等.响应面分析法优化杜仲叶中绿原酸水提工艺[J].安徽农业科学, 2011, 39 (7) :3965-3967, 3970.
[8]	李盈蕾, 陈建华, 孙吉佑, 等.响应面分析法优选黄花菜多糖提取工艺[J].中国酿造, 2010 (7) :72-74.