Abstract: The oral acute toxicity of 1, 8- cineole on mice was tested by the modified Karber method, and the oral accumulative toxicity was carried out by a fixed dose method.The results showed that the half-lethal dose ( LD50) of1, 8-cineole was 3847.34 mg /kg.bw with the 95% confidence interval of 3352.80 ~ 4414.82mg /kg.bw. The liver and kidney were the key targets organ of acute poisoning of 1, 8- cineole on mice. The accumulative toxicity test revealed that the accumulative coefficient K was larger than 5.No obviously influence of 1, 8- cineole on the feed conversion rate and organ coefficient of mice was observed compared to the control group ( p > 0.05) .1, 8-cineole exhibited no obviously harmful effects on organ of mice.1, 8-cineole displayed low acute toxicity and no obviously accumulative toxicity.