The oral acute and accumulative toxicity of 1, 8-cineole on mice
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摘要: 采用改良寇氏法测试了1,8-桉叶油素对小鼠的急性毒性,并采用固定剂量蓄积毒性系数法测试了其蓄积毒性。结果表明,1,8-桉叶油素对小鼠经口半数致死剂量(LD50)为3847.34mg/kg.bw,95%置信限为3352.804414.82mg/kg.bw,急性中毒主要靶器官为肝脏和肾脏;小鼠蓄积性毒性实验所得蓄积系数K>5,与对照组相比,1,8-桉叶油素对小鼠饲料利用率与脏器系数无明显影响(p>0.05),对脏器无明显不良影响。可见1,8-桉叶油素对小鼠属于低毒化学物质,无明显蓄积毒性。Abstract: The oral acute toxicity of 1, 8- cineole on mice was tested by the modified Karber method, and the oral accumulative toxicity was carried out by a fixed dose method.The results showed that the half-lethal dose ( LD50) of1, 8-cineole was 3847.34 mg /kg.bw with the 95% confidence interval of 3352.80 ~ 4414.82mg /kg.bw. The liver and kidney were the key targets organ of acute poisoning of 1, 8- cineole on mice. The accumulative toxicity test revealed that the accumulative coefficient K was larger than 5.No obviously influence of 1, 8- cineole on the feed conversion rate and organ coefficient of mice was observed compared to the control group ( p > 0.05) .1, 8-cineole exhibited no obviously harmful effects on organ of mice.1, 8-cineole displayed low acute toxicity and no obviously accumulative toxicity.
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Keywords:
- 1, 8-cineole /
- acute toxicity /
- accumulative toxicity
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[1] Ciftci O, Ozdemir I, Tanyildizi S, et al.Antioxidative effects of curcumin, β-myrcene and 1, 8-cineole against 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin-induced oxidative stress in rats liver[J].Toxicol Ind Health November, 2011, 27 (5) :447-453.
[2] Morcia C, Malnati M, Terzi V.In vitro antifungal activity of terpinen-4-ol, eugenol, carvone, 1, 8-cineole (eucalyptol) and thymol against mycotoxigenic plant pathogens[J].Food Additives&Contaminants:Part A, 2012, 29 (3) :415-422.
[3] Lika A, Rozman V, Kalinovi c'I, et al.Bioactivity of1, 8-cineole against red flour beetle, Tribolium Castaneum (Herbst) [J].Pupae.Poljoprivreda, 2011, 17 (1) :58-63.
[4] Bastos V P D, Gomes A S, Lima F J B, et al.Inhaled1, 8-cineole reduces inflammatory parameters in airways of ovalbumin-challenged guinea pigs[J].Basic&Clinical Pharmacology&Toxicology, 2011, 108 (1) :34-39.
[5] Takaishi M, Fujita F, Uchida K, et al.1, 8-cineole, a TRPM8agonist, is a novel natural antagonist of human TRPA1[J].Molecular Pain, 2012 (8) :86-98.
[6] Subongkot T, Opanasopit P, Rojanarata T, et al.Effect of limonene and 1, 8 cineole on the skin penetration of fluorescein sodium deformable liposomes[J].Advanced Materials Research, 2012, 506:449-452.
[7] Santos F A.Estudo farmacológico de 1, 8-cineole, umóxido terpênico presente emóleos essenciais de plantas[D].Fortaleza, CE, Brazil:Universidade Federal do Ceará.1999.
[8] 余永莉, 王传铜, 冯国纹, 等.桉叶油急性毒性与某些遗传毒性实验结果[J].毒理学杂志, 2010, 24 (6) :501-503. [9] 张宏, 李啸红, 余永莉, 等.桉叶油对小鼠胚胎发育影响的研究[J].时珍国医国药, 2011, 22 (5) :1155-1157. [10] 张笑乐, 向楠, 殷中琼, 等.油樟叶挥发油对兔的急性皮肤刺激、眼睛刺激和皮肤光毒性的研究[J].中国农学通报, 2011, 27 (23) :36-39. [11] 中华人民共和国卫生部.GB1593.1-15193.21食品安全性毒理学评价程序和方法[S].2003:19-31. [12] 李建科.食品毒理学[M].北京:中国剂量出版社, 2007, 138-143. [13] Vincenzi M De, Silano M, Vincenzi A De, et al.Constituents of aromatic plants:eucalyptol[J].Fitoterapia, 2002, 73 (3) :269-275.
[14] Lawler IR, Stapley J, Foley WJ, et al.Ecological example of conditioned flavor aversion in plant-herbivore interactions:Effect of terpenes of Eucalyptus leaves on feeding by common ringtail and brushtail possums[J].Journal of Chemical Ecology, 1999, 25 (2) :401-415.
[15] Kristiansen E, Madsen C.Induction of protein droplet (α2μ-globulin) nephropathy in male rats after short-term dosage with 1, 8-cineole and l-limonene[J].Toxicology Letters, 1995, 80 (1-3) :147-152.
[16] Lahlou S, Figueiredo A F, Magalhes PJC, et al.Cardiovascular effects of 1, 8-cineole, a terpenoid oxide present in many plant essential oils, in normotensive rats[J].Can.J.Physiol.Pharmacol, 2002, 80 (12) :1125-1131.
[17] Soares MCMS, Damiani CEN, Moreira CM, et al.Eucalyptol, an essential oil, reduces contractile activity in rat cardiac muscle[J].Brazilian Journal of Medical and Biological Research, 2005, 38 (3) :453-461.
[18] Mclean S, Boyle R R, Brandon S, et al.Pharmacokinetics of1, 8-cineole, a dietary toxin, in the brushtail possum (Trichosurus vulpecula) :Significance for feeding[J].Xenobiotica, 2007, 37 (9) :903-922.
[19] Santos F A, Rao V S N.1, 8-Cineol, a Food Flavoring Agent, Prevents Ethanol-Induced Gastric Injury in Rats[J].Digestive Diseases and Sciences, 2001, 46 (2) :331-337.
[20] Santos F A, Silva R M, ToméA R, et al.1, 8-Cineole protects against liver failure in an in-vivo murine model of endotoxemic shock[J].Journal of Pharmacy and Pharmacology, 2001, 53 (4) :505-511.
[21] Ciftci O, Ozdemir I, Tanyildizi S, et al.Antioxidative effects of curcumin, β-myrcene and 1, 8-cineole against 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin induced oxidative stress in rats liver[J].Toxicology and Industrial Health, 2011, 27 (5) :447-453.
[22] McLean SR, Brandon, S, Boyle RR, et al.Development of tolerance to the dietary plant secondary metabolite 1, 8-cineole by the Brushtail Possum (Trichosurus vulpecula) [J].Journal of Chemical Ecology, 2008, 34, (5) :672-680.
[23] Boyle RR, McLean S.Constraint of feeding by chronic ingestion of 1, 8-cineole in the brushtail possum (Trichosurus vulpecula) [J].Journal of Chemical Ecology, 2004, 30 (4) :757-775.
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