• EI
  • Scopus
  • 中国科技期刊卓越行动计划项目资助期刊
  • 北大核心期刊
  • DOAJ
  • EBSCO
  • 中国核心学术期刊RCCSE A+
  • 中国精品科技期刊
  • JST China
  • FSTA
  • 中国农林核心期刊
  • 中国科技核心期刊CSTPCD
  • CA
  • WJCI
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
中国精品科技期刊2020

大孔吸附树脂纯化水芹总黄酮的研究

陈况况, 章宏慧, 刘东红, 叶兴乾, 陈健初

陈况况, 章宏慧, 刘东红, 叶兴乾, 陈健初. 大孔吸附树脂纯化水芹总黄酮的研究[J]. 食品工业科技, 2013, (20): 246-250. DOI: 10.13386/j.issn1002-0306.2013.20.069
引用本文: 陈况况, 章宏慧, 刘东红, 叶兴乾, 陈健初. 大孔吸附树脂纯化水芹总黄酮的研究[J]. 食品工业科技, 2013, (20): 246-250. DOI: 10.13386/j.issn1002-0306.2013.20.069
CHEN Kuang-kuang, ZHANG Hong-hui, LIU Dong-hong, YE Xing-qian, CHEN Jian-chu. Research of the purification process of flavonoids in Oenanthe javanica by macroporous resins[J]. Science and Technology of Food Industry, 2013, (20): 246-250. DOI: 10.13386/j.issn1002-0306.2013.20.069
Citation: CHEN Kuang-kuang, ZHANG Hong-hui, LIU Dong-hong, YE Xing-qian, CHEN Jian-chu. Research of the purification process of flavonoids in Oenanthe javanica by macroporous resins[J]. Science and Technology of Food Industry, 2013, (20): 246-250. DOI: 10.13386/j.issn1002-0306.2013.20.069

大孔吸附树脂纯化水芹总黄酮的研究

基金项目: 

国家科技支撑计划项目(2012BAD31B02);

详细信息
    作者简介:

    陈况况 (1990-) , 女, 硕士研究生在读, 研究方向:功能性成分研究。;

  • 中图分类号: TS255.1

Research of the purification process of flavonoids in Oenanthe javanica by macroporous resins

  • 摘要: 通过比较六种大孔吸附树脂对水芹粗黄酮提取物静态吸附性能,筛选出大孔吸附树脂AB-8,并考察了动态吸附条件中的上样流速、洗脱溶剂浓度和体积及洗脱速率。结果表明,AB-8大孔吸附树脂对水芹黄酮的静态吸附率为71.37%,解吸率为76.57%,动态吸附优化条件为:上样流速为1mL/min,采用4BV 80%乙醇以1.5mL/min流速洗脱,黄酮含量为42.60mg的水芹粗提物纯度由7.1%提高到53.78%,树脂富集倍数7.6。通过HPLC初步分析,纯化前后黄酮种类基本不变,主要为芦丁、槲皮素-3-O-葡萄糖苷、槲皮素、山奈酚这四种成分,以HPLC测定的总黄酮含量为指标计算纯化前后水芹粗提物纯度分别为6.44%和55.65%,树脂富集倍数为8.65。 
    Abstract: By comparing static adsorption properties of flavonoids from the Oenanthe javanica extraction on six types of macroporous adsording resin, AB-8 had a higher adsorption capacity. The dynamic adsorption separating condition including loaded rate, ethanol concentration and amount of wash solvent, desorption velocity were studied. Results indicated that static adsorption rate for flavonoid from the Oenanthe javanica was71.37%, desorption rate was 76.57%. The optimized condition was loading rate 1mL/min, desorption rate 1.5mL/min, 4BV 80% ethanol as eluent.Under this condition the purity of the Oenanthe javanica extracts with 42.60mg flavone content was improved from 7.1% to 53.78% and the enrichment amount of AB-8 macroporous adsording resin was 7.6 multiple. High performance liquid chromatography (HPLC) analysis results showed that the flavonoids of Oenanthe javanica extracts before and after purified both included rutin, quercetin, quercetin-3-O-glucoside, kaempferol. Results of HPLC analysis indicated that the purity of Oenanthe javanica flavonoids was improved from 6.44% to 55.65% and the enrichment amount of AB-8 macroporous adsording resin was8.65 multiple.
  • [1] 王克勤.芹菜黄酮类物质的分离纯化与药理功能研究[D].长沙:湖南农业大学, 2009:111-123.
    [2] 郑瑞昌, 黄阿根, 钱建亚.水芹黄酮提取工艺的研究[J].扬州大学学报, 2001, 4 (4) :50-51.
    [3] 刘恒蔚, 高梦祥, 赵燕.野生水芹黄酮类物质的提取工艺[J].长江大学学报, 2006 (8) :188-190.
    [4] 任荣军.大孔树脂吸附技术在黄酮类成分分离纯化中的应用[J].中国药业, 2008, 17 (16) :71-73.
    [5]

    Jun L, Lou J, Yi S, et al.A simple method for the simultaneous decoloration and deproteinization of crude levan extract from Paenibacillus polymxa EJS-3 by macroporous resin[J].Bioresource Technology, 2010, 15 (101) :6077-6083.

    [6]

    Wang J, Wu F, Zhao H, et al.Isolation of flavonoids from mulberry (Morus alba L.) leaves with macroporos resins[J].African Journal of Biotechnology, 2008, 7 (13) :2147-2155.

    [7]

    Du H, Wang H, Yu J.Enrichment and purification of total flavonoid C-glycosides from abrus mollis extracts with macroporous resins[J].Industrial&Engineering Chemistry Research, 2012, 51:7349-7354.

    [8]

    Liu Y, Liu J, Chen X, et al.Preparative separation and purification of lycopene from tomato skins extracts by macroporous adsorption resins[J].Food Chemistry, 2010, 123:1027-1034.

    [9] 刘恒蔚, 高梦祥, 赵燕.野生水芹黄酮类物质的提取工艺[J].长江大学学报:自然科学版, 2009 (3) :188-190.
    [10] 毛飞, 顾茅俊, 杨坤.水芹总黄酮提取工艺正交实验研究[J].中国药物应用与监测, 2007 (4) :43-45.
    [11]

    Kim D O, Jeong S W, Lee C Y.Antioxidant capacity of phenolic phytochemicals from various cultivars of plums[J].Food Chemistry, 2003, 81 (3) :321-326.

    [12] 贾海伦.芹菜黄酮提取及分离纯化工艺研究[D].哈尔滨:黑龙江大学, 2008.
    [13]

    Li C, Zheng Y, Wang X.Simultaneous separation and purification of flavonoids and oleuropein from Olea europaea L. (olive) leaves using macroporous resin[J].Journal of Food Agriculture, 2011, 91:2826-2834.

    [14] 王克勤, 刘仲华, 罗军武, 等.XDA-1大孔树脂对芹菜黄酮分离纯化的研究[J].食品与机械, 2007, 23 (3) :65-68.
    [15] 彭燕.不同烹饪、配送方法对芹菜感官、营养和功能品质的影响[D].杭州:浙江大学, 2011.
    [16] 李春美, 钟朝辉, 窦宏亮, 等.大孔树脂分离纯化柚皮黄酮的研究[J].农业工程学报, 2006, 22 (3) :153-156.
    [17] 周英新.基于黄酮类分子构型的大孔树脂吸附动力学模型研究[D].北京:北京中医药大学, 2012.
    [18]

    Chen Z, Zhang A, Li J, et al.Study on the adsorption feature of rutin aqueous solution on macroporous adsorption resins[J].Journal of Physical Chemistry, 2010, 114:4841-4853.

    [19] 何春霞.苏力坦阿巴白克力.AB-8大孔树脂纯化欧洲鳞毛蕨总黄酮的研究[J].生物技术, 2007, 17 (2) :57-59.
计量
  • 文章访问数:  170
  • HTML全文浏览量:  21
  • PDF下载量:  168
  • 被引次数: 0
出版历程
  • 收稿日期:  2013-04-15

目录

    /

    返回文章
    返回
    x 关闭 永久关闭