Abstract: With retention rate and deproteinized rate of polysaccharide as comprehensive evaluation indexes, to determine the process conditions of two methods including Sevage, TDA- NBA by the uniform design experiment. Results showed that optimum conditions were as follows:Sevage and feed liquid volume rate was1 ∶2, chloroform and NBA volume rate was 3 ∶1, oscillating time was 34min, standing time was 36min, deproteinized rate retention rate and of polysaccharide were 85.92%±0.15%, 42.68%±0.10%, respectively. TDA and NBA volume rate was 1 ∶20, TDA-NBA and polysaccharide solution volume rate was 1 ∶2, oscillating time was 39min, standing time was 60min, deproteinized rate retention rate and of polysaccharide were 66.21% ±0.11%, 91.49%±0.30%, respectively. Results showed that TDA-NBA was better than Sevage.