响应面法优化热水浸提灰树花多糖的工艺研究

顾华杰; 孙燕; 李良智; 王桃云; 胡翠英; 袁红霞

doi:10.13386/j.issn1002-0306.2013.19.048

响应面法优化热水浸提灰树花多糖的工艺研究

苏州科技学院化学生物与材料工程学院

基金项目:

苏州科技学院基金资助项目(XKY201126)；苏州市科技支撑计划(SN201131)；苏州应用基础研究计划(SN201206)；

详细信息

作者简介:
顾华杰 (1981-) , 男, 硕士, 实验师, 研究方向:食用菌生理生化和天然产物提取。;

中图分类号: TQ914
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出版历程
- 收稿日期: 2013-03-06

Optimization of hot water extraction of polysaccharides from Grifola frondosa by response surface methodology

School of Chemistry Biological and Material Engineering, Suzhou University of Science and Technology

摘要

摘要: 以灰树花子实体为原料,采用响应面法对热水浸提灰树花多糖的工艺进行了优化。通过单因素实验确定了最佳提取次数为2次。利用Plackett-Burman设计确定了影响多糖得率的主要因素为浸提温度、料液比、浸提时间。再用最陡爬坡实验逼近最优响应区域。最后通过Box-Behnken设计建立了多糖得率的二次多项式模型,并获得了最优提取条件为:浸提温度85℃、浸提时间2.51h、料液比1∶25,经验证实验得到多糖得率为24.423%。
- 灰树花多糖 /
- 热水浸提法 /
- Plackett-Burman设计 /
- 最陡爬坡实验 /
- Box-Behnken设计
Abstract: In this experiment, the hot water extraction of polysaccharides from fruiting body of Grifola frondosa was optimized by response surface methodology.The appropriate times of extraction was determined to be twice by single-factor tests.It was found from the Plackett-Burman design that extraction temperature, ratio of material to liquid and extraction time were three major factors influencing the yield of GFP, and then the optimal response range was approached by the steepest ascent experiment.Lastly, a quadratic polynomial model relating the influencing factors and the yield was established through the Box-Behnken design, and the optimal conditions were: 85℃ of extraction temperature, 2.51h of extraction time, and 1 ∶ 25 of ratio of material to liquid.The yield of 24.423% was obtained by the verification experiment.
- Griflola frondosas polysaccharides /
- hot water extraction /
- Plackett-Burman design /
- steepest ascent experiment /
- Box-Behnken design

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参考文献(32)

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