微波法提取玉米须抗痛风活性组分的工艺研究

郭兵兵; 张燕; 赵文竹; 于志鹏; 安晓宁; 刘静波

doi:10.13386/j.issn1002-0306.2013.19.037

微波法提取玉米须抗痛风活性组分的工艺研究

1. 吉林大学军需科技学院
2. 吉林大学生物与农业工程学院

基金项目:

吉林省科技发展重点项目(YYZX201123-3)；长春市科技计划项目(11KZ52)；

详细信息

作者简介:
郭兵兵 (1990-) , 女, 本科, 研究方向:营养与功能食品。;

中图分类号: TQ461
计量
- 文章访问数: 154
- HTML全文浏览量: 25
- PDF下载量: 222
出版历程
- 收稿日期: 2013-03-07

Study on microwave extraction of anti-gout active substance from corn silk

College of Quartermaster Technology, Jilin University
College of Biological and Agricultural Engineering, Jilin University

摘要

摘要: 为了优化玉米须中抗痛风活性组分提取工艺,在单因素实验的基础上,采用二次回归正交旋转组合设计,以乙醇浓度、微波功率、微波时间和液料比为考察因素,以黄酮提取率和黄嘌呤氧化酶(XOD)抑制率为指标,优化微波萃取玉米须中抗痛风活性组分的最佳工艺。结果表明:最佳工艺条件为:微波功率600W,乙醇浓度为70%,萃取时间10min,液料比20∶1(mL/g)。在此条件下测得酶抑制率为39%,黄酮提取率为26%。
- 抗痛风活性 /
- 玉米须 /
- 微波萃取 /
- 黄酮类化合物
Abstract: Anti-gout active substance from corn silk was carried out using microwave extraction.The Quadric Regression Orthogonal Rotary Test was employed to screen the key factors which would affect the extraction yield and the inhibition ratio of the anti-gout active substance from corn silk.Key factors were the concentration of ethanol, the microwave extracting power, the irradiation time and the liquid-solid ratio.The optimized condition within the experimental range of the variables studied were: microwave of 600W, ethanol concentration of 70%, irradiation time of 10min and the solvent to material ratio was 20∶1 (mL /g) .Under this condition, the extraction yield of flavonoids was 26% and the enzyme inhibition ratio was 39%.
- anti-gout /
- corn silk /
- microwave extraction /
- flavonoids

HTML全文

参考文献(18)

[1]	孙爱宁, 张日, 吴德沛, 等.内科学[M].北京:人民卫生出版社, 2004.
[2]	朱旭.玉米须抗痛风活性成分的筛选[D].长春:长春中医药大学, 2009.
[3]	So Alexander.Developments in the scientific and clinical understanding of gout[J].Arthritis research&therapy, 2008, 10 (5) :221-226.
[4]	Corradoa, D‘onofriof, Santoron, et al.Pathogenesis, clinical findings and management of acute and chronic gout[J].Minerva, 2006, 97 (6) :495-509.
[5]	Choi HK, Curhan G.Coffee, Tea, and caffeine consumption and serum uric acid level[J].Arthritis&Rheumatism, 2007, 57 (5) :816-821.
[6]	夏道宗, 张英, 吕圭源.黄酮类化合物防治高尿酸血症和痛风的研究进展[J].中国药学杂志, 2009 (10) :721-723.
[7]	Nguyen MT, Awale S, Tezuka Y, et al.Xanthine oxidase inhibitory activity of Vietnamese medicinal plants[J].Biol Pharm Bull, 2004 (9) :1414-1421.
[8]	邱智东.玉米须提取物及其制备方法在制备治疗痛风药物中的作用[P].中国:101444599A.2009-06-03.
[9]	Xiao WH, Ham LJ, Shi B, et al.Microwave-assisted extraction of flavonoids from Radix Astragali[J].Separation and Purification Technology, 2008, 62:614-618.
[10]	Noro T, Oda Y, Miyase T, et al.Inhibition of xanthine oxidase from the flowers and buds of daphne genkwa[J].Chem Pharm Bull, 1983, 31:3984-3987.
[11]	Liu BG, Zhu YY.Extraction of flavonoids from flavonoid-rich parts in tartary buckwheat and identification of the main flavonoids[J].Journal of Food Engineering, 2007, 78:584-587.
[12]	Zhao WZ, Yu ZH, Liu JB, et al.Optimization of Microwave Extraction of Polysaccharides from Stigma Maydis by Central Composition Design[J].African Journal of Food Science and Technology, 2011, 2 (7) :157-166.
[13]	Zhao WZ, Yu ZH, Liu JB, et al.Optimized extraction of polysaccharides from corn silk by pulsed electric field and response surface quadratic design[J].Journal of the Science of Food and Agriculture, 2011 (12) :2201-2209.
[14]	Liu J, Wang CN, Wang ZZ, et al.The antioxidant and free-radical scavenging activities of extract and fractions from corn silk (Zea mays L.) and related flavone glycosides[J].Food Chemistry, 2011, 126:261-269.
[15]	王翠娜, 刘静波, 王二雷, 等.玉米须食品化及分析研究进展[J].食品科学, 2010, 31 (15) :304-307.
[16]	刘军, 刘静波, 王作昭, 等.玉米须黄酮类化合物构效及合成基因调控研究进展[J].食品科学, 2010, 31 (13) :316-320.
[17]	Umamaheswari M, Asokkumar k, Somasundaram A, et al.Xanthine oxidase inhibitory activity of some Indian medical plants[J].Journal of Ethnopharmacology, 2007, 109:547-551.
[18]	Chen HJ, Chung CP, Chiang W, et al.Anti-inflammatory effects and chemical study of a flavonoid-enriched fraction from adlay bran[J].Food Chemistry, 2011, 126:1741-1748.