响应面分析法优化蓝靛果花色苷纯化工艺研究

基金项目:

哈尔滨市科技攻关计划项目(2011HG6AN107)；

详细信息

Optimization of purification technique of anthocyanin from Lonicera Edulis via response surface methodology (RSM)

摘要: 利用响应面法对蓝靛果花色苷的纯化工艺进行优化。在单因素实验基础上,选取上样浓度、径长比、洗脱流速为自变量,以花色苷的纯度为响应值,利用Box-Benhnken中心组合设计原理和响应面分析法,研究各自变量及其交互作用对纯度的影响,获得的最佳工艺条件为:上样浓度2.5mg/mL、径长比1∶25、洗脱剂浓度为70%的乙醇、洗脱流速3mL/min,获得的花色苷的纯度为19.54%。
- 蓝靛果 /
- 花色苷 /
- X-5 /
- 纯化 /
- 纯度 /
- 响应面
Abstract: Response surface methodology (RSM) was used to optimize purification of anthocyanin from Lonicera edulis.Based on single factor tests, three purification parameters including sample concentration, ratio of diameter to length, elution flow rate were optimized through Box-Benhnken central combination design and RSM for achieving maximum the purification of anthocyanin.The interaction of the respective variables and their influence on the purification were studied to obtain the optimum purification conditions of anthocyanin were as follows:the sample concentration 2.5mg/mL, diameter to length ratio 1∶25, eluent concentration 70% ethanol, elution velocity 3mL/min.Under such conditions, the purity of anthocyanins was up to 19.54%.
- Lonicera edulis /
- anthocyanin /
- X-5 /
- purification /
- purity /
- response surface methodology (RSM)

[1]	向延菊, 王大伟.蓝靛果忍冬的研究利用现状及其发展前景[J].塔里木农垦大学学报, 2004, 16 (4) :26-29.
[2]	Zholobova z P.Basis for commercial cultivation of blue honeysuckle[J].Sadovodstvo I Vinogradarstvo, 1990 (8) :23-25.
[3]	任健.稠李属果实色素理化性质及及抗氧化抗疲劳作用研究[D].哈尔滨:东北林业大学, 2011.
[4]	李文星.蓝靛果花色苷提取及其抗肿瘤功能机理的初步研究[D].哈尔滨:东北林业大学, 2011.
[5]	杨玲.蓝靛果提取物抗氧化及抗癌作用的研究[D].哈尔滨:东北林业大学, 2009.
[6]	Nadia A, Soliman Mahmoud M, Berekaa Yasser R.Polyglutamic acid (PGA) production by Bacillus sp.SAB-26:application of Plackett-Burman experimental design to evaluate culture requirements[J].Appl Microbiol Biotechnol, 2005, 69:259-267.
[7]	Chen Xiong, Chen Shouwen, Sun Ming, et al.Mediumoptimization by response surface methodology for polygammaglutamic acid production using dairy manure as the basis of a solid substrate[J].Appl Microbiol Biotechnol, 2005, 69:390-396.
[8]	唐琳, 李子江.两种pH法测定玫瑰花花色苷含量的比较[J].食品科学, 2009, 30 (18) :310-313.
[9]	李蕊.野生浆果花色苷提取纯化、结构鉴定及体外抗氧化活性的分析[D].哈尔滨:东北林业大学, 2011.
[10]	田福.三种野生浆果花色苷的提取、纯化及抗氧化活性研究[D].哈尔滨:东北林业大学, 2008.