响应面分析法优化蓝靛果花色苷纯化工艺研究

基金项目:

哈尔滨市科技攻关计划项目(2011HG6AN107)；

详细信息

Optimization of purification technique of anthocyanin from Lonicera Edulis via response surface methodology (RSM)

摘要: 利用响应面法对蓝靛果花色苷的纯化工艺进行优化。在单因素实验基础上,选取上样浓度、径长比、洗脱流速为自变量,以花色苷的纯度为响应值,利用Box-Benhnken中心组合设计原理和响应面分析法,研究各自变量及其交互作用对纯度的影响,获得的最佳工艺条件为:上样浓度2.5mg/mL、径长比1∶25、洗脱剂浓度为70%的乙醇、洗脱流速3mL/min,获得的花色苷的纯度为19.54%。
- 蓝靛果 /
- 花色苷 /
- X-5 /
- 纯化 /
- 纯度 /
- 响应面
Abstract: Response surface methodology (RSM) was used to optimize purification of anthocyanin from Lonicera edulis.Based on single factor tests, three purification parameters including sample concentration, ratio of diameter to length, elution flow rate were optimized through Box-Benhnken central combination design and RSM for achieving maximum the purification of anthocyanin.The interaction of the respective variables and their influence on the purification were studied to obtain the optimum purification conditions of anthocyanin were as follows:the sample concentration 2.5mg/mL, diameter to length ratio 1∶25, eluent concentration 70% ethanol, elution velocity 3mL/min.Under such conditions, the purity of anthocyanins was up to 19.54%.
- Lonicera edulis /
- anthocyanin /
- X-5 /
- purification /
- purity /
- response surface methodology (RSM)

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