响应面法优化醇提荷叶总黄酮和生物碱的工艺研究

霍丹群; 冯丹; 曾娜; 代宇; 周荣灵; 侯长军

doi:10.13386/j.issn1002-0306.2013.18.034

响应面法优化醇提荷叶总黄酮和生物碱的工艺研究

1. 重庆大学生物工程学院
2. 国家固态酿造工程技术研究中心,泸州老窖股份有限公司

基金项目:

国家自然科学基金(81102132)；四川省科技支撑计划(2010NZ0093)；酿酒生物技术及应用四川省重点实验室开放基金(NJ2011-03)；中央高校研究生创新基金(CDJXS102300)；重庆大学大型仪器设备开放基金；

详细信息

作者简介:
霍丹群 (1965-) , 女, 博士, 教授, 主要从事微生物资源开发与利用、生物大分子的结构与功能等方面的研究。;

中图分类号: TQ914
计量
- 文章访问数: 0118
- HTML全文浏览量: 012
- PDF下载量: 0309
出版历程
- 收稿日期: 2013-03-17

Study on the optimization of ethanol extraction of total flavonoids and alkaloids from lotus leaf using response surface methodology

College of Bioengineering, Chongqing University
National Engineering Research Center of Solid-State Brewing Luzhou Laojiao Group Co., Ltd.

摘要

摘要: 采用超声-酶-渗漉协同方法醇提荷叶总黄酮和生物碱,以总黄酮和生物碱的总评值为评价指标,用Box-Behnken响应面设计考察因素对其的影响,对结果进行二项式拟合及响应曲面分析得到最适宜提取条件为:乙醇浓度75%、料液比1∶26（g/mL）、超声时间80min、纤维素酶添加量14mg/g、渗漉速度3mL/min、渗漉液收集体积为9倍,在此条件下荷叶总黄酮和生物碱得率分别为6.7735%和0.7817%,与理论预测值偏差为-3%左右。实验结果表明,优化后的提取工艺稳定可行,且具有得率高、提取时间短等优点。
- 荷叶 /
- 总黄酮 /
- 总生物碱 /
- 响应面设计法
Abstract: The ethanol extraction process of lotus leaf total flavonoids and alkaloids was studied by ultrasonicenzyme-diacolation collaborative extraction technique.Box-Behnken response surface design method was used to investigate the effect of influence factors on overall desirability.The experimental data were analysed using second-order polynomial equation and 3D-response surface graphs.The optimum extraction conditions were as follows :ethanol concentration 75%, solid/liquid ratio 1∶26 (g/mL) , ultrasound time 80min, cellulase dosage 14mg/g, collecting 9 folds of percolate at a rate of 3mL/min, the proportion of total flavonoids and alkaloids was 6.7735% and 0.7817% under this condtions, respectively.The deviation between observed and predicted values of yield was-3%.The results indicated that the optimized extraction technique was a new method of higher efficiency and less time consumption.
- lotus leaf /
- total flavonoids /
- alkaloids /
- response surface method

HTML全文

参考文献(19)

[1]	国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社, 2010:258-259.
[2]	Fisher M, Gokhman I, Pick U, et al.A salt-resistant plasma membrane carbonic anhydrase is induced by salt in Dunaliella salina[J].The Journal of Biological Chemistry, 1996, 271 (30) :17718-17723.
[3]	王福刚, 曹娟, 刘斌, 等.荷叶化学成分及其药理作用研究进展[J].时珍医药国药, 2010, 21 (9) :2339-2340.
[4]	Kashiwada Y, Aoshima A, Ikeshiro Y, et al.Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure-activity correlations with related alkaloids[J].Bioorganic Medicinal Chemistry, 2005, 13 (2) :443-448.
[5]	张燕, 李俊.荷叶醇提物对脑缺血再灌注抗氧化作用的实验研究[J].食品与药品, 2012, 14 (1) :29-31.
[6]	谢文, 王凯民.荷叶降脂护肝汤治疗脂肪肝的临床研究[J].中国医药导报, 2009, 28 (6) :41-42.
[7]	朱燕超, 陶文沂.超声波辅助提取荷叶总黄酮工艺的研究[J].应用化工, 2007, 36 (11) :1106-1108.
[8]	康璇, 王在贵, 高雪英, 等.荷叶黄酮的提取工艺研究[J].安徽农业科学, 2009, 37 (23) :11179-11180.
[9]	赵俊, 王洪章, 高敏燕, 等.荷叶中荷叶碱提取工艺的研究[J].中草药, 2003, 34 (10) :916-917.
[10]	蒋益虹.荷叶生物碱的提取工艺优化[J].浙江大学学报, 2004, 30 (5) :519-523.
[11]	邓胜国, 邓泽元.紫外分光光度法测定荷叶总黄酮含量[J].南昌大学学报:理科版, 2008, 32 (2) :148-150.
[12]	王伟, 谭晓梅.荷叶总生物碱含量测定方法的研究[J].中药材, 2004, 27 (1) :50-52.
[13]	于浩, 阮美娟.响应面法优化荷叶黄酮超声提取条件的研究[J].食品研究与开发, 2010, 31 (1) :55-60.
[14]	Gupta S, Manohar CS.An improved response surfacemethod for the determination of failure probability and importance measures[J].Structural Safety, 2004, 26:123-132.
[15]	王福刚, 曹娟.星点设计-效应面法优选荷叶总生物碱超声提取工艺[J].中草药, 2011, 34 (4) :616-619.
[16]	苏柘僮, 刘英.应用Box-Behnken设计优化地榆皂苷的闪式提取工艺研究[J].中草药, 2012, 43 (3) :501-504.
[17]	伍勇, 贺福元, 曹燕, 等.星点设计-效应面优化法对四物汤醇沉工艺的研究[J].中国医药导报, 2009, 83 (6) :17-19.
[18]	伍永富, 吴品江.Box-Behnken设计-效应面法优化木犀草素-β-环糊精包合物的制备工艺研究[J].中草药, 41 (7) :1094-1099.
[19]	吴伟, 崔光华, 陆彬.实验设计中多指标的优化星点设计和总评“归一值”的应用[J].中国药学杂志, 2000, 35 (8) :530-533.