响应面法优化醇提荷叶总黄酮和生物碱的工艺研究
Study on the optimization of ethanol extraction of total flavonoids and alkaloids from lotus leaf using response surface methodology
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摘要: 采用超声-酶-渗漉协同方法醇提荷叶总黄酮和生物碱,以总黄酮和生物碱的总评值为评价指标,用Box-Behnken响应面设计考察因素对其的影响,对结果进行二项式拟合及响应曲面分析得到最适宜提取条件为:乙醇浓度75%、料液比1∶26（g/mL）、超声时间80min、纤维素酶添加量14mg/g、渗漉速度3mL/min、渗漉液收集体积为9倍,在此条件下荷叶总黄酮和生物碱得率分别为6.7735%和0.7817%,与理论预测值偏差为-3%左右。实验结果表明,优化后的提取工艺稳定可行,且具有得率高、提取时间短等优点。Abstract: The ethanol extraction process of lotus leaf total flavonoids and alkaloids was studied by ultrasonicenzyme-diacolation collaborative extraction technique.Box-Behnken response surface design method was used to investigate the effect of influence factors on overall desirability.The experimental data were analysed using second-order polynomial equation and 3D-response surface graphs.The optimum extraction conditions were as follows :ethanol concentration 75%, solid/liquid ratio 1∶26 (g/mL) , ultrasound time 80min, cellulase dosage 14mg/g, collecting 9 folds of percolate at a rate of 3mL/min, the proportion of total flavonoids and alkaloids was 6.7735% and 0.7817% under this condtions, respectively.The deviation between observed and predicted values of yield was-3%.The results indicated that the optimized extraction technique was a new method of higher efficiency and less time consumption.
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