液相色谱-串联质谱法测定植物油中黄曲霉毒素B<sub>1</sub>的含量

甘勇强

doi:10.13386/j.issn1002-0306.2013.18.026

液相色谱-串联质谱法测定植物油中黄曲霉毒素B₁的含量

甘勇强

广西壮族自治区食品药品检验所

详细信息

作者简介:
甘勇强 (1963-) , 男, 副主任药师, 研究方向:食品药品安全。;

中图分类号: TS227
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出版历程
- 收稿日期: 2013-03-26

Determination of Aflatoxin B1 in vegetable oil by LC-MS/MS

GAN Yong-qiang

Guangxi Institute of Food and Drug Control

摘要

摘要: 目的:建立植物油中黄曲霉毒素B1含量的液相质谱-串联质谱测定方法。方法:采用液相色谱-串联质谱法（LC-MS/MS）,以乙腈为溶剂提取植物油中黄曲霉毒素B1。ZORBAX SB-C18（2.1mm×50mm,1.8μm）色谱柱,流动相为乙腈-0.2%甲酸（40∶60）梯度洗脱,流速为0.3mL/min,进样量为5μL。采用电喷雾离子化四极杆串联质谱,多反应监测方式测定样品的浓度。检测离子对分别为m/z 313.0→285.0和m/z 313.0→241.0。结果:黄曲霉毒素B1进样量在0.204⁴.08ng/mL（r=0.9991）范围内呈良好的线性关系,平均加样回收率为98.15%,RSD=1.12%（n=9）。结论:该法简便快捷、结果准确,可用于植物油中黄曲霉毒素B1的含量的测定。
- 植物油 /
- 液相色谱-串联质谱法 /
- 黄曲霉毒素B1
Abstract: Objective:To establish an LC-MS/MS method to determine the content of Aflatoxin B1 in vegetable oil.Methods:Aflatoxin B1 was extracted from vegetable oil by acetonitrile.The analysis was performed on a ZORBAX SB-C18 column.The mobile phase consisted of acetonitrile-0.2% methane acid solution for gradient elution.The flow rate was 0.3mL/min.Then 5μL was injected for analysis, Electrospray ionization (ESI) sources was applied and operated in the position ion mode.Multiple reaction monitoring (MRM) mode with the transition of m/z 313.0→285.0 and m/z 313.0→240.0 were used to quantify Aflatoxin B1.Results:The sample was in good linear at the range of 0.204⁴.08ng/mL (r=0.9991) .The average recovery rate was 98.15% (n=9) .The RSD was 1.12 %.Conclusion : The method was simple, quick and accurate, proved to be applyable in the content determination of Aflatoxin B1 in vegetable oil.
- vegetable oil /
- LC-MS/MS /
- Aflatoxin B1

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参考文献(7)

[1]	刘旭, 张雪梅, 党献民, 等.用油中黄曲霉毒素B1高效低耗检测技术研究[J].中国油脂, 2011, 37 (12) :43-46.
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[4]	宫小明, 任一平, 董静, 等.超高效液相色谱串联质谱法测定花生、粮油中18种真菌毒素[J].分析测试学报, 2011, 30 (1) :6-12.
[5]	杨静, 哈益明, 王锋.高效液相色谱-串联质谱法检测花生中的黄曲霉毒素B1[J].分析实验室, 2009, 28 (6) :35-38.
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