Process optimization for extracting superoxide dismutase from waste wine yeast
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摘要: 以葡萄酒泥酵母试材,采用甲苯破壁法分离纯化超氧化物歧化酶(SOD),选择甲苯用量、作用时间、作用温度以及缓冲液pH进行单因素实验,通过L9(34)正交实验优化工艺参数。结果表明:在甲苯用量0.8mL/g,作用时间25min,作用温度50℃,磷酸缓冲液pH8.0的最优条件下,分离纯化的SOD平均比活力为178.73U/mg。此方法操作简单,成本低,适合工厂化生产。Abstract: Waste wine yeast as the research object, the superoxide dismutase (SOD) was extracted from it by toluene method.Four extraction parameters including amount of toluene, react time, react temperature and pH used for single factor test, and optimization of process parameters through L9 (34) orthogonal test.The results showed that the optimal amount of toluene were 0.8mL /g, react time was 25min, react temperature was 50℃, pH was 8.0, under these conditions, the specific activity of SOD was 178.73U /mg.The process was simple and inexpensive and coude be applied to industrialized production.
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