β-聚苹果酸/明胶微胶囊的制备
详细信息Preparation of microcapsules based on poly malic acid and gelatin
-
摘要: 文章对β-聚苹果酸（β-poly malic acid,β-PMA）和明胶（gelatin）通过复凝聚法制备微胶囊进行了描述。在常温常压下,通过Plackett-Burman设计得出主要的影响因素是β-PMA和明胶的浓度、明胶的pH、反应时间。根据Box-Behnke设计得出了较好的制备条件,在该模型下优化得出的最佳条件为:PMLA浓度0.1%,明胶浓度0.13%,明胶pH4.0,反应时间25min时,微胶囊粒径为288.37nm。
-
关键词:
- β-聚苹果酸; /
- 明胶; /
- Box-Behnken; /
- 微胶囊;
Abstract: This paper described the preparation of novel biodegradable and edible nanocapsule based on self-assembly of β-Polymalic acid ( β-PMA) and gelatin at normal temperature and pressure.After the Plackett-Burman design ( PBD) by Design-Expert 7.0, it was found that the size and PDI of the nanocapsule measured by Malvern zetasizer Nano-ZS instrument depended on mass concentration of β-PMA and gelatin, pH of gelatin and reaction time.A Box-Behnken design ( BBD ) was used to optimize the preparation conditions of the β-PMA / gelatin nanocapsule, and then the best experimental condition was obtained as follows: the concentrations of β-PMA and gelatin were 0.1% and 0.13% , pH of gelatin was 4.0, reaction time was 25min. The size of the nanocapsules prepared under the best contidions was 288.37nm. -
[1] Shimada K, Matsushima K, Fukumoto J, et al.Poly-L-malic acid:a new protease inhibitor from Penicillium cuclopium[J].Biochem BiopHys Res Commun, 1969, 35 (5) :619-624.
[2] 李睿颖, 许勤虎, 刘晓鸥, 等.聚苹果酸及其衍生物的研究与发展[J].食品工程, 2009 (2) :6-9. [3] Nagata N, Nakahara T, Tabuchi T.Fermentative production of poly ( (-malic acid) , a polyelectrolytic biopolyester, by Aureobasidium sp[J].Biosci Biotech Biochem, 1993, 57 (4) :638-642.
[4] 戴种, 李云政, 张青山, 等.生物可降解材料聚苹果酸的研究进展[J].材料导报, 2005, 19 (5) :359-262. [5] 刘双江.出芽短梗霉菌株CBS591.75和DSM2404发酵生产聚苹果酸的研究[J].生物工程学报, 1997, 13 (3) :279-283. [6] 宋长江, 孙村民, 等.同时获得副产物普鲁兰的β-聚苹果酸的制备方法[P].中国:CN101100687A.2008-01-09. [7] 徐志南, 张慧莉, 等.一种产生聚苹果酸的菌株及利用其发酵生产聚苹果酸的方法[P].中国:CN102220248A.2011-10-19. [8] 许时婴, 张晓鸣, 夏书琴, 等.《微胶囊技术——原理与应用》.北京:化学工业出版社, 2006:60-78. [9] 高艳, 欧阳建勋, 谢定, 等.复合凝聚法制备辣椒素微胶囊的研究[J].粮食科技与经济, 2011, 36 (5) :51-53. [10] Zsolt Keresztessy, Magdolna Bodnár, Elizabeth Ber, et al.elf-assembling chitosan/poly-γ-glutamic acid nanoparticles for targeted drug delivery[J].Colloid Polym Sci, 2009, 28 (7) :759-765.
[11] 徐向宏, 何明珠.《实验设计与Design-Expert、SPSS应用》.北京:科学出版社, 2010:146-160;194-204. [12] 鞠蕾, 马霞.γ-聚谷氨酸的生物合成及其纳米胶囊的制备[D].上海:上海应用技术学院, 2012:21-23. [13] 刘建峰, 葛向阳, 梁云祥.响应面法优化豆粕固态发酵工艺的研究[J].中国生物工程杂志, 2007, 27 (6) :87-91. [14] 代文亮, 程龙, 陶文沂.响应面法在紫杉醇产生菌发酵前提优化中的应用[J].中国生物工程杂志, 2007, 27 (11) :66-72. [15] 陈海华, 鲁军.响应面发优化狭鳕鱼皮明胶的微波辅助提取工艺[J].食品与发酵工业, 2009, 35 (8) :168-174.
计量
- 文章访问数: 180
- HTML全文浏览量: 18
- PDF下载量: 166
下载:
